Rabbit anti-Human ASF1A Polyclonal Antibody | anti-ASF1A antibody

ASF1A, CT (ASF1A, Histone chaperone ASF1A, Anti-silencing function protein 1 homolog A, CCG1-interacting factor A) (PE)

Cat. Num. AAA6275621

• Gene Names: ASF1A; CIA; CGI-98; HSPC146
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ASF1A; Polyclonal Antibody; CT (ASF1A; Histone chaperone ASF1A; Anti-silencing function protein 1 homolog A; CCG1-interacting factor A) (PE); CCG1-interacting factor A; anti-ASF1A antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-ASF1A antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: ASF1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 139-168 amino acids from the C-terminal region of human ASF1A.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(ASF1A Antibody (C-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the ASF1A antibody detected the ASF1A protein (arrow).)

Related Product Information for anti-ASF1A antibody

This gene encodes a member of the H3/H4 family of histone chaperone proteins and is similar to the anti-silencing function-1 gene in yeast. The protein is a key component of a histone donor complex that functions in nucleosome assembly. It interacts with histones H3 and H4, and functions together with a chromatin assembly factor during DNA replication and repair. [provided by RefSeq].

Product Categories/Family for anti-ASF1A antibody

Antibodies; Histones

References

Jasencakova, Z., et al. Mol. Cell 37(5):736-743(2010). Newton-Cheh, C., et al. Nat. Genet. 41(4):399-406(2009). Banumathy, G., et al. Mol. Cell. Biol. 29(3):758-770(2009). Ambagala, A.P., et al. J. Virol. 83(1):200-209(2009). Pilyugin, M., et al. PLoS ONE 4 (12), E8328 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 7661592
• NCBI GeneID: 25842
• NCBI Accession #: NP_054753.1
• NCBI GenBank Nucleotide #: NM_014034.2
• UniProt Accession #: Q9Y294; Q6IA08; Q9P014
• Molecular Weight: 22,969 Da
• NCBI Official Full Name: histone chaperone ASF1A
• NCBI Official Synonym Full Names: anti-silencing function 1A histone chaperone
• NCBI Official Symbol: ASF1A
• NCBI Official Synonym Symbols: CIA; CGI-98; HSPC146
• NCBI Protein Information: histone chaperone ASF1A; hCIA; hAsf1; hAsf1a; CCG1-interacting factor A; ASF1 anti-silencing function 1 homolog A; anti-silencing function protein 1 homolog A
• UniProt Protein Name: Histone chaperone ASF1A
• Protein Family: Histone chaperone
• UniProt Gene Name: ASF1A
• UniProt Synonym Gene Names: hAsf1; hAsf1a; CIA; hCIA
• UniProt Entry Name: ASF1A_HUMAN

NCBI Description

This gene encodes a member of the H3/H4 family of histone chaperone proteins and is similar to the anti-silencing function-1 gene in yeast. The protein is a key component of a histone donor complex that functions in nucleosome assembly. It interacts with histones H3 and H4, and functions together with a chromatin assembly factor during DNA replication and repair. [provided by RefSeq, Jul 2008]

Uniprot Description

ASF1A: Histone chaperone that facilitates histone deposition and histone exchange and removal during nucleosome assembly and disassembly. Cooperates with chromatin assembly factor 1 (CAF-1) to promote replication-dependent chromatin assembly and with HIRA to promote replication-independent chromatin assembly. Required for the formation of senescence-associated heterochromatin foci (SAHF) and efficient senescence-associated cell cycle exit. Interacts with histone H3 (including both histone H3.1 and H3.3) and histone H4. Interacts with the CHAF1A, CHAF1B and RBBP4 subunits of the CAF-1 complex. Interacts with CABIN1, HAT1, HIRA, NASP, TAF1, TLK1, TLK2 and UBN1. Ubiquitously expressed. Belongs to the ASF1 family.

Protein type: DNA repair, damage; Histone-binding

Chromosomal Location of Human Ortholog: 6q22.31

Cellular Component: nucleoplasm; protein complex; nuclear chromatin; nucleus

Molecular Function: protein binding; histone binding; chromatin binding

Biological Process: osteoblast differentiation; nucleosome assembly; muscle cell differentiation; transcription, DNA-dependent; negative regulation of chromatin silencing; DNA replication-independent nucleosome assembly; chromatin modification; DNA repair; DNA replication-dependent nucleosome assembly