Western Blot (WB)
(Western Blot analysis of PFKFB3 on different cell lysates using anti-PFKFB3 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: PC-12 Lane 3: A431)
Rabbit PFKFB3 Polyclonal Antibody | anti-PFKFB3 antibody
PFKFB3 Polyclonal Antibody
Gene Names
PFKFB3; PFK2; IPFK2; iPFK-2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, Flow Cytometry, Functional Assay, Immunoprecipitation
Purity
ProA affinity purified
Synonyms
PFKFB3; Polyclonal Antibody; PFKFB3 Polyclonal Antibody; 6PF-2-K/Fru-2; 6-P2ase 3; PFK/FBPase 3; 6 phosphofructo 2 kinase/ fructose 2; 6 bisphosphatase; 6 phosphofructo 2 kinase/fructose 2; 6 biphosphatase 3; 6-bisphosphatase; 6-P2ASE brain/placenta-type isozyme; 6PF 2 K/Fru 2; 6 P2ASE brain/placenta type isozyme; 6PF 2-K/Fru 2; 6 P2ase 3; F263; fructose 6 phosphate; 2 kinase/fructose 2; Fructose-2; Inducible 6 phosphofructo 2 kinase/fructose 2; iPFK 2; iPFK-2; IPFK2; PFK2; Renal carcinoma antigen NY REN 56; Renal carcinoma antigen NY-REN-56; uPFK 2; anti-PFKFB3 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
PFKFB3 polyclonal antibody detects endogenous levels of PFKFB3 protein.
Purity/Purification
ProA affinity purified
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
500
Applicable Applications for anti-PFKFB3 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunoprecipitation (IP)
Application Notes
WB: 1:500-1:1,000
ICC: 1:50-1?200
IHC: 1:50-1?200
FC/FACS: 1:50-1:100
ICC: 1:50-1?200
IHC: 1:50-1?200
FC/FACS: 1:50-1:100
Immunogen
Recombinant protein
Preparation and Storage
Store at 4 degree C after thawing.
Aliquot store at -20 degree C or -80 degree C.
Avoid repeated freeze/thaw cycles.
Aliquot store at -20 degree C or -80 degree C.
Avoid repeated freeze/thaw cycles.
Western Blot (WB)
(Western Blot analysis of PFKFB3 on different cell lysates using anti-PFKFB3 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: PC-12 Lane 3: A431)
Western Blot (WB)
(Western Blot analysis of PFKFB3 on different cell lysates using anti-PFKFB3 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: PC-12 Lane 3: A431)
Immunocytochemistry (ICC)
(ICC staining PFKFB3 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemistry (ICC)
(ICC staining PFKFB3 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Related Product Information for anti-PFKFB3 antibody
Among the enzymes playing role in glycolysis, four allosteric PFKFB enzymes 1-4 expressed by four independent PFKFB genes, catalyze the rate-limiting phosphorylation of fructose-6-phosphate to fructose-1, 6-bisphosphate, using ATP as the energy source in the glycolysis pathway. Among these four allosteric enzymes, PFKFB3 enzyme retains the highest Kinase/Biphosphatase activity ratio and is expressed by PFKFB3 gene which has been demonstrated to be highly expressed in leukemic cells and in solid tumors. Moreover, mitogenic, hypoxic and inflammatory conditions have an inductive effect on the expression of PFKFB3. Hence upregulation of PFKFB genes specific to cancer cells compared to their normal counterparts (from the same patients) with more robust over-expression in breast and lung cancer make it a more appropriate target.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 isoform 2
NCBI Official Synonym Full Names
6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3
NCBI Official Symbol
PFKFB3
NCBI Official Synonym Symbols
PFK2; IPFK2; iPFK-2
NCBI Protein Information
6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3
UniProt Protein Name
6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3
Protein Family
UniProt Gene Name
PFKFB3
UniProt Synonym Gene Names
6PF-2-K/Fru-2,6-P2ase 3
UniProt Entry Name
F263_HUMAN
NCBI Description
The protein encoded by this gene belongs to a family of bifunctional proteins that are involved in both the synthesis and degradation of fructose-2,6-bisphosphate, a regulatory molecule that controls glycolysis in eukaryotes. The encoded protein has a 6-phosphofructo-2-kinase activity that catalyzes the synthesis of fructose-2,6-bisphosphate (F2,6BP), and a fructose-2,6-biphosphatase activity that catalyzes the degradation of F2,6BP. This protein is required for cell cycle progression and prevention of apoptosis. It functions as a regulator of cyclin-dependent kinase 1, linking glucose metabolism to cell proliferation and survival in tumor cells. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2016]
Research Articles on PFKFB3
Similar Products
Product Notes
The PFKFB3 pfkfb3 (Catalog #AAA3013243) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PFKFB3 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PFKFB3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunoprecipitation (IP). WB: 1:500-1:1,000 ICC: 1:50-1?200 IHC: 1:50-1?200 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the PFKFB3 pfkfb3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PFKFB3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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