Human Aldolase B ELISA Kit | aldolase B elisa kit

Human Aldolase B ELISA Kit

Cat. Num. AAA7606316

• Gene Names: ALDOB; ALDB; ALDO2
• Reactivity: Human
• Synonyms: Aldolase B; Human Aldolase B ELISA Kit; aldolase B elisa kit

• Reactivity: Human
• Sequence Length: 364

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.469-30ng/ml
• Sensitivity: 0.281ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for aldolase B elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- aldolase B antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-aldolase B antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the aldolase B amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of aldolase B can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 113611
• NCBI GeneID: 229
• UniProt Accession #: P05062; Q13741; Q13742; Q5T7D6
• Molecular Weight: 39,473 Da
• NCBI Official Full Name: Fructose-bisphosphate aldolase B
• NCBI Official Synonym Full Names: aldolase, fructose-bisphosphate B
• NCBI Official Symbol: ALDOB
• NCBI Official Synonym Symbols: ALDB; ALDO2
• NCBI Protein Information: fructose-bisphosphate aldolase B
• UniProt Protein Name: Fructose-bisphosphate aldolase B
• UniProt Gene Name: ALDOB
• UniProt Synonym Gene Names: ALDB

NCBI Description

Fructose-1,6-bisphosphate aldolase (EC 4.1.2.13) is a tetrameric glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Vertebrates have 3 aldolase isozymes which are distinguished by their electrophoretic and catalytic properties. Differences indicate that aldolases A, B, and C are distinct proteins, the products of a family of related 'housekeeping' genes exhibiting developmentally regulated expression of the different isozymes. The developing embryo produces aldolase A, which is produced in even greater amounts in adult muscle where it can be as much as 5% of total cellular protein. In adult liver, kidney and intestine, aldolase A expression is repressed and aldolase B is produced. In brain and other nervous tissue, aldolase A and C are expressed about equally. There is a high degree of homology between aldolase A and C. Defects in ALDOB cause hereditary fructose intolerance. [provided by RefSeq, Dec 2008]

Uniprot Description

ALDOB: Defects in ALDOB are the cause of hereditary fructose intolerance (HFI). HFI is an autosomal recessive disease that results in an inability to metabolize fructose and related sugars. Complete exclusion of fructose results in dramatic recovery; however, if not treated properly, HFI subjects suffer episodes of hypoglycemia, general ill condition, and risk of death the remainder of life. Belongs to the class I fructose-bisphosphate aldolase family.

Protein type: Carbohydrate Metabolism - fructose and mannose; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - pentose phosphate pathway; EC 4.1.2.13; Lyase

Chromosomal Location of Human Ortholog: 9q31.1

Cellular Component: cytosol; microtubule organizing center

Molecular Function: ATPase binding; cytoskeletal protein binding; fructose-bisphosphate aldolase activity; identical protein binding; protein binding

Biological Process: fructose 1,6-bisphosphate metabolic process; fructose metabolic process; gluconeogenesis; glycolysis; NADH oxidation; positive regulation of ATPase activity

Disease: Fructose Intolerance, Hereditary