Rabbit PFDN5 Polyclonal Antibody | anti-PFDN5 antibody

PFDN5 Antibody (Center)

Cat. Num. AAA9214024

• Gene Names: PFDN5; MM1; MM-1; PFD5
• Reactivity: Human, mouse (Predicted Reactivity: Bovine)
• Applications: Western Blot, ELISA, Immunohistochemistry
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: PFDN5; Polyclonal Antibody; PFDN5 Antibody (Center); Prefoldin subunit 5; C-Myc-binding protein Mm-1; Myc modulator 1; MM1; PFD5; anti-PFDN5 antibody

• Host: Rabbit
• Reactivity: Human, mouse (Predicted Reactivity: Bovine)
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This PFDN5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 79-108 amino acids from the Central region of human PFDN5.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Vial Concentration: 0.5 (varies by lot)
• Sequence Positions: 79-108
• Sequence Length: 154

• Applicable Applications for anti-PFDN5 antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)
• Application Notes: WB~~1:1000
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(PFDN5 Antibody (Center) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the PFDN5 antibody detected the PFDN5 protein (arrow).)

Western Blot (WB)

(PFDN5 Antibody (Center) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the PFDN5 antibody detected the PFDN5 protein (arrow).)

Immunohistochemistry (IHC)

(PFDN5 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PFDN5 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-PFDN5 antibody

This gene encodes a member of the prefoldin alpha subunit
family. The encoded protein is one of six subunits of prefoldin, a
molecular chaperone complex that binds and stabilizes newly
synthesized polypeptides, thereby allowing them to fold correctly.
The complex, consisting of two alpha and four beta subunits, forms
a double beta barrel assembly with six protruding coiled-coils. The
encoded protein may also repress the transcriptional activity of
the proto-oncogene c-Myc. Alternatively spliced transcript variants
encoding different isoforms have been described. [provided by
RefSeq].

Product Categories/Family for anti-PFDN5 antibody

Signal Transduction

References

Ma, H.C., et al. J. Biomed. Sci. 15(4):417-425(2008)
Yoshida, T., et al. Exp. Cell Res. 314(6):1217-1228(2008)
Lubyova, B., et al. J. Biol. Chem. 282(44):31944-31953(2007)
Hagio, Y., et al. J. Cell. Biochem. 97(1):145-155(2006)
Bruneel, A., et al. Proteomics 5(15):3876-3884(2005)

NCBI and Uniprot Product Information

• NCBI GI #: 22202633
• NCBI GeneID: 5204
• NCBI Accession #: NP_002615.2; NP_665904.1
• NCBI GenBank Nucleotide #: NM_002624.3
• UniProt Accession #: Q99471; Q54AA8; Q9C083; Q9C084; A8K9A8
• Molecular Weight: 17328
• NCBI Official Full Name: prefoldin subunit 5 isoform alpha
• NCBI Official Synonym Full Names: prefoldin subunit 5
• NCBI Official Symbol: PFDN5
• NCBI Official Synonym Symbols: MM1; MM-1; PFD5
• NCBI Protein Information: prefoldin subunit 5
• UniProt Protein Name: Prefoldin subunit 5
• Protein Family: Prefoldin
• UniProt Gene Name: PFDN5
• UniProt Synonym Gene Names: MM1; PFD5
• UniProt Entry Name: PFD5_HUMAN

NCBI Description

This gene encodes a member of the prefoldin alpha subunit family. The encoded protein is one of six subunits of prefoldin, a molecular chaperone complex that binds and stabilizes newly synthesized polypeptides, thereby allowing them to fold correctly. The complex, consisting of two alpha and four beta subunits, forms a double beta barrel assembly with six protruding coiled-coils. The encoded protein may also repress the transcriptional activity of the proto-oncogene c-Myc. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

PFDN5: Binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. Binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins. Represses the transcriptional activity of MYC. Belongs to the prefoldin subunit alpha family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 12q12

Cellular Component: cytoplasm; nucleus; prefoldin complex

Molecular Function: protein binding; unfolded protein binding; transcription corepressor activity

Biological Process: 'de novo' posttranslational protein folding; cellular protein metabolic process; protein folding; regulation of transcription, DNA-dependent; retina development in camera-type eye; negative regulation of transcription, DNA-dependent