Rabbit anti-Human, Mouse PFDN5 Polyclonal Antibody | anti-PFDN5 antibody

PFDN5, ID (PFDN5, MM1, PFD5, Prefoldin subunit 5, C-Myc-binding protein Mm-1, Myc modulator 1) (Biotin)

Cat. Num. AAA6332872

• Gene Names: PFDN5; MM1; MM-1; PFD5
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PFDN5; Polyclonal Antibody; ID (PFDN5; MM1; PFD5; Prefoldin subunit 5; C-Myc-binding protein Mm-1; Myc modulator 1) (Biotin); Myc modulator 1; anti-PFDN5 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PFDN5. Species Crossreactivity: mouse
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-PFDN5 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 79-108 in the central region of human PFDN5, UniProt Accession #NP_002615.2; Q99471. Species sequence homology: bovine
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in Jurkat cell line lysates (35ug/lane) usingMBS6013634. This demonstrates that MBS6013634 detected the PFDN5 protein (arrow).)

Western Blot (WB)

(Western Blot analysis in mouse heart tissue lysates (35ug/lane) usingMBS6013634. This demonstrates that MBS6013634 detected the PFDN5 protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue using MBS6013634 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS6013634 for immunohistochemistry)

Related Product Information for anti-PFDN5 antibody

This gene encodes a member of the prefoldin alpha subunit family. The encoded protein is one of six subunits of prefoldin, a molecular chaperone complex that binds and stabilizes newly synthesized polypeptides, thereby allowing them to fold correctly. The complex, consisting of two alpha and four beta subunits, forms a double beta barrel assembly with six protruding coiled-coils. The encoded protein may also repress the transcriptional activity of the proto-oncogene c-Myc. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq].

Product Categories/Family for anti-PFDN5 antibody

Antibodies; Cancer Markers

References

1. Ma, H.C., et al. J. Biomed. Sci. 15(4):417-425(2008). 2. Yoshida, T., et al. Exp. Cell Res. 314(6):1217-1228(2008). 3. Lubyova, B., et al. J. Biol. Chem. 282(44):31944-31953(2007). 4. Hagio, Y., et al. J. Cell. Biochem. 97(1):145-155(2006). 5. Bruneel, A., et al. Proteomics 5(15):3876-3884(2005).

NCBI and Uniprot Product Information

• NCBI GI #: 22202633
• NCBI GeneID: 5204
• NCBI Accession #: NP_002615.2
• NCBI GenBank Nucleotide #: NM_002624.3
• UniProt Accession #: Q99471; Q54AA8; Q9C083; Q9C084; A8K9A8
• Molecular Weight: 12,379 Da
• NCBI Official Full Name: prefoldin subunit 5 isoform alpha
• NCBI Official Synonym Full Names: prefoldin subunit 5
• NCBI Official Symbol: PFDN5
• NCBI Official Synonym Symbols: MM1; MM-1; PFD5
• NCBI Protein Information: prefoldin subunit 5; c-myc binding protein; myc modulator-1
• UniProt Protein Name: Prefoldin subunit 5
• Protein Family: Prefoldin
• UniProt Gene Name: PFDN5
• UniProt Synonym Gene Names: MM1; PFD5
• UniProt Entry Name: PFD5_HUMAN

NCBI Description

This gene encodes a member of the prefoldin alpha subunit family. The encoded protein is one of six subunits of prefoldin, a molecular chaperone complex that binds and stabilizes newly synthesized polypeptides, thereby allowing them to fold correctly. The complex, consisting of two alpha and four beta subunits, forms a double beta barrel assembly with six protruding coiled-coils. The encoded protein may also repress the transcriptional activity of the proto-oncogene c-Myc. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

PFDN5: Binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. Binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins. Represses the transcriptional activity of MYC. Belongs to the prefoldin subunit alpha family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 12q12

Cellular Component: cytoplasm; nucleus; prefoldin complex

Molecular Function: protein binding; unfolded protein binding; transcription corepressor activity

Biological Process: 'de novo' posttranslational protein folding; cellular protein metabolic process; regulation of transcription, DNA-dependent; protein folding; retina development in camera-type eye; negative regulation of transcription, DNA-dependent