Rabbit anti-Human PFDN4 Polyclonal Antibody | anti-PFDN4 antibody

PFDN4, NT (PFDN4, PFD4, Prefoldin subunit 4, Protein C-1) (APC)

Cat. Num. AAA6332860

• Gene Names: PFDN4; C1; PFD4
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PFDN4; Polyclonal Antibody; NT (PFDN4; PFD4; Prefoldin subunit 4; Protein C-1) (APC); Protein C-1; anti-PFDN4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-PFDN4 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: PFDN4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 4-32 amino acids from the N-terminal region of human PFDN4.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(PFDN4 Antibody (N-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the PFDN4 antibody detected the PFDN4 protein (arrow).)

Related Product Information for anti-PFDN4 antibody

This gene encodes a member of the prefoldin beta subunit family. The encoded protein is one of six subunits of prefoldin, a molecular chaperone complex that binds and stabilizes newly synthesized polypeptides, thereby allowing them to fold correctly. The complex, consisting of two alpha and four beta subunits, forms a double beta barrel assembly with six protruding coiled-coils.

Product Categories/Family for anti-PFDN4 antibody

Antibodies; Proteins

References

Simons, C.T., et al. J. Biol. Chem. 279(6):4196-4203(2004). Martin-Benito, J., et al. EMBO J. 21(23):6377-6386(2002). Hartl, F.U., et al. Science 295(5561):1852-1858(2002). Deloukas, P., et al. Nature 414(6866):865-871(2001). Cowan, N.J., et al. Nat. Struct. Biol. 6(11):990-991(1999).

NCBI and Uniprot Product Information

• NCBI GI #: 12408677
• NCBI GeneID: 5203
• NCBI Accession #: NP_002614.2
• NCBI GenBank Nucleotide #: NM_002623.3
• UniProt Accession #: Q9NQP4; Q5TD11; Q92779
• Molecular Weight: 15,314 Da
• NCBI Official Full Name: prefoldin subunit 4
• NCBI Official Synonym Full Names: prefoldin subunit 4
• NCBI Official Symbol: PFDN4
• NCBI Official Synonym Symbols: C1; PFD4
• NCBI Protein Information: prefoldin subunit 4; prefoldin 4; protein C-1
• UniProt Protein Name: Prefoldin subunit 4
• Protein Family: Prefoldin
• UniProt Gene Name: PFDN4
• UniProt Synonym Gene Names: PFD4
• UniProt Entry Name: PFD4_HUMAN

NCBI Description

This gene encodes a member of the prefoldin beta subunit family. The encoded protein is one of six subunits of prefoldin, a molecular chaperone complex that binds and stabilizes newly synthesized polypeptides, thereby allowing them to fold correctly. The complex, consisting of two alpha and four beta subunits, forms a double beta barrel assembly with six protruding coiled-coils. [provided by RefSeq, Jul 2008]

Uniprot Description

PFDN4: Binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. Binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins. Belongs to the prefoldin subunit beta family.

Chromosomal Location of Human Ortholog: 20q13.2

Cellular Component: mitochondrion; cytoplasm; nucleus; cytosol; prefoldin complex

Molecular Function: protein binding; chaperone binding; unfolded protein binding

Biological Process: 'de novo' posttranslational protein folding; cellular protein metabolic process; protein folding