Rabbit anti-Human ORC2L Polyclonal Antibody | anti-ORC2L antibody

ORC2L, ID (ORC2, ORC2L, Origin recognition complex subunit 2) (Biotin)

Cat. Num. AAA6329605

• Gene Names: ORC2; ORC2L
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ORC2L; Polyclonal Antibody; ID (ORC2; Origin recognition complex subunit 2) (Biotin); ORC2; Origin recognition complex subunit 2; anti-ORC2L antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-ORC2L antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: ORC2L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 207-237 amino acids from the Central region of human ORC2L.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(ORC2L Antibody (Center) western blot analysis in HUVEC cell line lysates (35ug/lane).This demonstrates the ORC2L antibody detected the ORC2L protein (arrow).)

Related Product Information for anti-ORC2L antibody

The origin recognition complex (ORC) is a highly conserved six subunits protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. The protein encoded by this gene is a subunit of the ORC complex. This protein forms a core complex with ORC3,-4, and-5. It also interacts with CDC45 and MCM10, which are proteins known to be important for the initiation of DNA replication. This protein has been demonstrated to specifically associate with the origin of replication of Epstein-Barr virus in human cells, and is thought to be required for DNA replication from viral origin of replication. Alternatively spliced transcript variants have been found, one of which is a nonsense-mediated mRNA decay candidate.

Product Categories/Family for anti-ORC2L antibody

Antibodies; Proteins

References

Huen, M.S., et al. Cell 131(5):901-914(2007). Siddiqui, K., et al. J. Biol. Chem. 282(44):32370-32383(2007). Olsen, J.V., et al. Cell 127(3):635-648(2006). Radichev, I., et al. J. Biol. Chem. 281(32):23264-23273(2006). Teer, J.K., et al. J. Biol. Chem. 281(10):6253-6260(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 5453830
• NCBI GeneID: 4999
• NCBI Accession #: NP_006181.1
• NCBI GenBank Nucleotide #: NM_006190.4
• UniProt Accession #: Q13416; Q13204; Q53TX5
• Molecular Weight: 65,972 Da
• NCBI Official Full Name: origin recognition complex subunit 2
• NCBI Official Synonym Full Names: origin recognition complex, subunit 2
• NCBI Official Symbol: ORC2
• NCBI Official Synonym Symbols: ORC2L
• NCBI Protein Information: origin recognition complex subunit 2; origin recognition complex protein 2 homolog; origin recognition complex, subunit 2 homolog
• UniProt Protein Name: Origin recognition complex subunit 2
• UniProt Gene Name: ORC2
• UniProt Synonym Gene Names: ORC2L
• UniProt Entry Name: ORC2_HUMAN

NCBI Description

The origin recognition complex (ORC) is a highly conserved six subunits protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. The protein encoded by this gene is a subunit of the ORC complex. This protein forms a core complex with ORC3, -4, and -5. It also interacts with CDC45 and MCM10, which are proteins known to be important for the initiation of DNA replication. This protein has been demonstrated to specifically associate with the origin of replication of Epstein-Barr virus in human cells, and is thought to be required for DNA replication from viral origin of replication. Alternatively spliced transcript variants have been found, one of which is a nonsense-mediated mRNA decay candidate. [provided by RefSeq, Oct 2010]