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Western Blot (WB) (Western blotAll lanes: Periostin antibody at 2ug/mlLane 1:mouse liver tissueLane 2:Hela whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 94,88,84,91,81 kDaObserved band size: 93 kDa)

Rabbit anti-Human Periostin Polyclonal Antibody | anti-POSTN antibody

Rabbit anti-human Periostin polyclonal Antibody

Gene Names
POSTN; PN; OSF2; OSF-2; PDLPOSTN
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Caprylic Acid Ammonium Sulfate Precipitation purified
Synonyms
Periostin; Polyclonal Antibody; Rabbit anti-human Periostin polyclonal Antibody; Osteoblast-specific factor 2 POSTN OSF2; anti-POSTN antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Caprylic Acid Ammonium Sulfate Precipitation purified
Form/Format
Liquid
Sequence Length
779
Applicable Applications for anti-POSTN antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
Recommended dilution: WB:1:500-2000
Immunogen
Recombinant human Periostin protein
Conjugation
Non-conjugated
Storage Buffer
Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-49479 / sc-49480 / sc-67233
Preparation and Storage
Shipped at 4 degree C. Upon delivery, aliquot and store at -20 degree C or -80 degree C.

Western Blot (WB)

(Western blotAll lanes: Periostin antibody at 2ug/mlLane 1:mouse liver tissueLane 2:Hela whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 94,88,84,91,81 kDaObserved band size: 93 kDa)

Western Blot (WB) (Western blotAll lanes: Periostin antibody at 2ug/mlLane 1:mouse liver tissueLane 2:Hela whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 94,88,84,91,81 kDaObserved band size: 93 kDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human thymus tissue using MBS7002434 at dilution of 1:100)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human thymus tissue using MBS7002434 at dilution of 1:100)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human kidney tissue using MBS7002434 at dilution of 1:100)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human kidney tissue using MBS7002434 at dilution of 1:100)

Immunofluorescence (IF)

(Immunofluorescent analysis of Hela cells using MBS7002434 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

Immunofluorescence (IF) (Immunofluorescent analysis of Hela cells using MBS7002434 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Related Product Information for anti-POSTN antibody
Enhances incorporation of BMP1 in the fibronectin matrix of connective tissues, and subsequent proteolytic activation of lysyl oxidase LOX By similarity. Induces cell attachment and spreading and plays a role in cell adhesion. May play a role in extracellular matrix mineralization.
Product Categories/Family for anti-POSTN antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
80,357 Da
NCBI Official Full Name
periostin isoform 2
NCBI Official Synonym Full Names
periostin, osteoblast specific factor
NCBI Official Symbol
POSTN
NCBI Official Synonym Symbols
PN; OSF2; OSF-2; PDLPOSTN
NCBI Protein Information
periostin
UniProt Protein Name
Periostin
Protein Family
UniProt Gene Name
POSTN
UniProt Synonym Gene Names
OSF2; PN; OSF-2
UniProt Entry Name
POSTN_HUMAN

NCBI Description

This gene encodes a secreted extracellular matrix protein that functions in tissue development and regeneration, including wound healing, and ventricular remodeling following myocardial infarction. The encoded protein binds to integrins to support adhesion and migration of epithelial cells. This protein plays a role in cancer stem cell maintenance and metastasis. Mice lacking this gene exhibit cardiac valve disease, and skeletal and dental defects. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2015]

Uniprot Description

POSTN: Binds to heparin. Induces cell attachment and spreading and plays a role in cell adhesion. May play a role in extracellular matrix mineralization. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Cell adhesion; Motility/polarity/chemotaxis; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 13q13.3

Cellular Component: extracellular matrix; extracellular space; neuromuscular junction; proteinaceous extracellular matrix; trans-Golgi network

Molecular Function: cell adhesion molecule binding; heparin binding; protein binding

Biological Process: cell adhesion; extracellular matrix organization and biogenesis; negative regulation of cell-matrix adhesion; positive regulation of smooth muscle cell migration; regulation of Notch signaling pathway; regulation of systemic arterial blood pressure; response to estradiol stimulus; response to hypoxia; response to mechanical stimulus; response to muscle activity; skeletal development

Research Articles on POSTN

Similar Products

Product Notes

The POSTN postn (Catalog #AAA7002434) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-human Periostin polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Periostin can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). Recommended dilution: WB:1:500-2000. Researchers should empirically determine the suitability of the POSTN postn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Periostin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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