Rabbit anti-Human PENK Polyclonal Antibody | anti-PENK antibody

PENK Antibody (Center)

Cat. Num. AAA9213692

• Reactivity: Human
• Applications: Western Blot, ELISA, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: PENK; Polyclonal Antibody; PENK Antibody (Center); Proenkephalin-A; Synenkephalin; Met-enkephalin; Opioid growth factor; OGF; PENK(114-133); PENK(143-183); Met-enkephalin-Arg-Gly-Leu; Leu-enkephalin; PENK(237-258); Met-enkephalin-Arg-Phe; anti-PENK antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This PENK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 145-174 amino acids from the Central region of human PENK.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Vial Concentration: 0.5 (varies by lot)
• Sequence Positions: 145-174
• Sequence Length: 267

• Applicable Applications for anti-PENK antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: WB~~1:1000
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(PENK Antibody (Center) western blot analysis in T47D cell line lysates (35ug/lane).This demonstrates the PENK antibody detected the PENK protein (arrow).)

Immunohistochemistry (IHC)

(PENK antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PENK antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(PENK Antibody (Center) flow cytometric analysis of T47D cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-PENK antibody

Immunology; Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 208879446
• NCBI GeneID: 5179
• NCBI Accession #: NP_001129162.1; NP_006202.1
• NCBI GenBank Nucleotide #: NM_001135690.1
• UniProt Accession #: P01210; Q6FHC6; Q6FHE6; B2RC23
• Molecular Weight: 30787
• NCBI Official Full Name: proenkephalin-A preproprotein
• NCBI Official Synonym Full Names: proenkephalin
• NCBI Official Symbol: PENK
• NCBI Protein Information: proenkephalin-A
• UniProt Protein Name: Proenkephalin-A
• Protein Family: Proenkephalin
• UniProt Gene Name: PENK
• UniProt Synonym Gene Names: OGF
• UniProt Entry Name: PENK_HUMAN

NCBI Description

This gene encodes a preproprotein that is proteolytically processed to generate multiple protein products. These products include the pentapeptide opioids Met-enkephalin and Leu-enkephalin, which are stored in synaptic vesicles, then released into the synapse where they bind to mu- and delta-opioid receptors to modulate the perception of pain. Other non-opioid cleavage products may function in distinct biological activities. [provided by RefSeq, Jul 2015]

Uniprot Description

PENK: Met- and Leu-enkephalins compete with and mimic the effects of opiate drugs. They play a role in a number of physiologic functions, including pain perception and responses to stress. PENK(114-133) and PENK(237-258) increase glutamate release in the striatum. PENK(114-133) decreases GABA concentration in the striatum. Belongs to the opioid neuropeptide precursor family.

Protein type: Secreted; Hormone; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 8q23-q24

Cellular Component: extracellular region

Molecular Function: neuropeptide hormone activity; protein binding; opioid peptide activity

Biological Process: behavioral fear response; startle response; neuropeptide signaling pathway; locomotory behavior; signal transduction; sensory perception of pain