Western Blot (WB)
(Western blot analysis of lysates from CEM, rat L6 cell line and human liver tissue lysate(from left to right), using PEMT Antibody (N-term). MBS9211349 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
Rabbit anti-Human, mouse PEMT Polyclonal Antibody | anti-PEMT antibody
PEMT Antibody (N-term)
IHC-P~~1:10~50
Western Blot (WB)
(Western blot analysis of lysates from CEM, rat L6 cell line and human liver tissue lysate(from left to right), using PEMT Antibody (N-term). MBS9211349 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
Western Blot (WB)
(Western blot analysis of lysates from CEM, rat L6 cell line and human liver tissue lysate(from left to right), using PEMT Antibody (N-term). MBS9211349 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
Western Blot (WB)
(Western blot analysis of anti-PEMT Antibody (N-term) Pab MBS9211349 in mouse kidney tissue lysates (35ug/lane). PEMT(arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of anti-PEMT Antibody (N-term) Pab MBS9211349 in mouse kidney tissue lysates (35ug/lane). PEMT(arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PEMT antibody (N-term) MBS9211349, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PEMT antibody (N-term) MBS9211349, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Shields D.J., Biochim. Biophys. Acta 1532:105-114(2001).
Hu R.-M., Proc. Natl. Acad. Sci. U.S.A. 97:9543-9548(2000).
NCBI and Uniprot Product Information
NCBI Description
Phosphatidylcholine (PC) is the most abundant mammalian phospholipid. This gene encodes an enzyme which converts phosphatidylethanolamine to phosphatidylcholine by sequential methylation in the liver. Another distinct synthetic pathway in nucleated cells converts intracellular choline to phosphatidylcholine by a three-step process. The protein isoforms encoded by this gene localize to the endoplasmic reticulum and mitochondria-associated membranes. Alternate splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, May 2012]
Uniprot Description
PEMT iso2: Catalyzes three sequential methylation reactions of phosphatidylethanolamine (PE) by AdoMet, thereby producing phosphatidylcholine (PC). Belongs to the PEMT/PEM2 methyltransferase family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Endoplasmic reticulum; EC 2.1.1.17; Membrane protein, integral; Lipid Metabolism - glycerophospholipid; Membrane protein, multi-pass; EC 2.1.1.71; Mitochondrial; Methyltransferase
Chromosomal Location of Human Ortholog: 17p11.2
Cellular Component: endoplasmic reticulum membrane; mitochondrial membrane; integral to membrane
Molecular Function: phosphatidyl-N-methylethanolamine N-methyltransferase activity; phosphatidylethanolamine N-methyltransferase activity
Biological Process: methylation; cell proliferation; phospholipid metabolic process; glycerophospholipid biosynthetic process; phosphatidylcholine biosynthetic process; lipid metabolic process
Research Articles on PEMT
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Product Notes
The PEMT pemt (Catalog #AAA9211349) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PEMT Antibody (N-term) reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PEMT can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry-paraffin (IHC-p). WB~~1:1000 IHC-P~~1:10~50. Researchers should empirically determine the suitability of the PEMT pemt for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PEMT, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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