Rabbit anti-Mouse, Rat PED/PEA-15 Polyclonal Antibody | anti-PEA15 antibody

PED/PEA-15, CT (15kD Phosphoprotein Enriched in Astrocytes, Phosphoprotein Enriched in Astrocytes 15kD, Phosphoprotein Enriched in Astrocytes 15, PEA15, PEA-15 Protein, PED, Astrocytic Phosphoprotein PEA-15, HMAT1, HUMMAT1H, MAT1, MAT1H)

Cat. Num. AAA643353

• Reactivity: Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: PED/PEA-15; Polyclonal Antibody; CT (15kD Phosphoprotein Enriched in Astrocytes; Phosphoprotein Enriched in Astrocytes 15kD; Phosphoprotein Enriched in Astrocytes 15; PEA15; PEA-15 Protein; PED; Astrocytic Phosphoprotein PEA-15; HMAT1; HUMMAT1H; MAT1; MAT1H); Anti -PED/PEA-15; anti-PEA15 antibody

• Host: Rabbit
• Reactivity: Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PEA15. Species Crossreactivity: Mouse, rat.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, (without Mg2+, Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.

• Applicable Applications for anti-PEA15 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Suitable for use in Immunofluorescence, ELISA, Western Blot andImmunohistochemistry.; Dilution: Immunofluorescence: 1:100-1:500; ELISA: 1:40,000; Western Blot: 1:500-1:1000; Immunohistochemistry (Paraffin): 10ug/ml
• Immunogen: Synthetic peptide corresponding to human PEA15 at C-Terminal.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(PEA15(10ug/ml) was used in IHC to stain formalin-fixed, paraffin-embedded human kidney, tubules, followed by biotinylated goat,-rabbit IgG, alkaline phosphatase-streptavidin and chromogen.)

Western Blot (WB)

(IWestern blot analysis of extracts from Jurkat cells, treated with PMA 125ng/ml 30', using PEA-15. The lane on the right is treated with the synthesized peptide.)

Immunofluorescence (IF)

(Immunofluorescence analysis of HeLa cells, using PEA-15.The picture on the right is treated with the synthesized peptide.)

Related Product Information for anti-PEA15 antibody

PEA15 is a death effector domain (DED)-containing protein predominantly expressed in the central nervous system, particularly in astrocytes.

Product Categories/Family for anti-PEA15 antibody

Antibodies; Abs to Apoptosis

NCBI and Uniprot Product Information

• NCBI GI #: 2196870
• UniProt Accession #: Q15121; O00511
• Molecular Weight: 15,040 Da
• NCBI Official Full Name: Protein Enriched in Diabetes
• UniProt Protein Name: Astrocytic phosphoprotein PEA-15
• Protein Family: Astrocytic phosphoprotein
• UniProt Gene Name: PEA15
• UniProt Synonym Gene Names: PED
• UniProt Entry Name: PEA15_HUMAN

NCBI Description

This gene encodes a death effector domain-containing protein that functions as a negative regulator of apoptosis. The encoded protein is an endogenous substrate for protein kinase C. This protein is also overexpressed in type 2 diabetes mellitus, where it may contribute to insulin resistance in glucose uptake. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]

Uniprot Description

PEA-15: an ubiquitously expressed anti-apoptotic protein. Inhibits both Fas- and Tnfrsf1a/Tnfr1-mediated caspase-8 activity and apoptosis. Interacts with CASP8/FLICE and FADD. Regulates glucose transport by controlling both the content of GLUT1 glucose transporters on the plasma membrane and the insulin-dependent trafficking of GLUT4 from the cell interior to the surface. Most abundant in tissues such as heart, brain, muscle and adipose tissue which utilize glucose as an energy source. Lower expression in glucose-producing tissues. Higher levels of expression are found in tissues from individuals with type 2 diabetes than in controls.

Protein type: Motility/polarity/chemotaxis; Apoptosis

Chromosomal Location of Human Ortholog: 1q21.1

Cellular Component: microtubule associated complex; cytoplasm

Molecular Function: protein binding

Biological Process: negative regulation of glucose import; transport; apoptosis; carbohydrate transport; DNA damage checkpoint; response to morphine