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Western Blot (WB) (Western blotAll lanes: PDXK antibody at 5.63ug/mlLane 1: mouse liver tissueLane 2: Hela whole cell lysateLane 3: mouse kidney tissueLane 4: HepG2 whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 36,32,27 kDaObserved band size: 35 kDa)

Rabbit anti-Human, Mouse PDXK Polyclonal Antibody | anti-PDXK antibody

PDXK Antibody

Gene Names
PDXK; PKH; PNK; PRED79; C21orf97; HEL-S-1a; C21orf124
Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Antigen Affinity Purified
Synonyms
PDXK; Polyclonal Antibody; PDXK Antibody; Pyridoxal kinase; Pyridoxine kinase; C21orf124; C21orf97; PKH; PNK; PRED79; anti-PDXK antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid; PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Sequence Length
312
Applicable Applications for anti-PDXK antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB:1:1000-1:5000
IHC:1:20-1:200
Species
Human
Immunogen
Recombinant Human Pyridoxal kinase protein (1-312AA)
Conjugate
Non-conjugated
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-50961 / sc-67361
Immunogen Species
Homo sapiens (Human)
Research Area
Signal Transduction
Target Names
PDXK
Preparation and Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Western Blot (WB)

(Western blotAll lanes: PDXK antibody at 5.63ug/mlLane 1: mouse liver tissueLane 2: Hela whole cell lysateLane 3: mouse kidney tissueLane 4: HepG2 whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 36,32,27 kDaObserved band size: 35 kDa)

Western Blot (WB) (Western blotAll lanes: PDXK antibody at 5.63ug/mlLane 1: mouse liver tissueLane 2: Hela whole cell lysateLane 3: mouse kidney tissueLane 4: HepG2 whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 36,32,27 kDaObserved band size: 35 kDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human breast cancer using MBS7043630 at dilution 1:100)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human breast cancer using MBS7043630 at dilution 1:100)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human colon cancer using MBS7043630 at dilution 1:100)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human colon cancer using MBS7043630 at dilution 1:100)
Related Product Information for anti-PDXK antibody
Required for synthesis of pyridoxal-5-phosphate from vitamin B6.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
pyridoxal kinase
NCBI Official Synonym Full Names
pyridoxal (pyridoxine, vitamin B6) kinase
NCBI Official Symbol
PDXK
NCBI Official Synonym Symbols
PKH; PNK; PRED79; C21orf97; HEL-S-1a; C21orf124
NCBI Protein Information
pyridoxal kinase
UniProt Protein Name
Pyridoxal kinase
Protein Family
UniProt Gene Name
PDXK
UniProt Synonym Gene Names
C21orf124; C21orf97; PKH; PNK
UniProt Entry Name
PDXK_HUMAN

NCBI Description

The protein encoded by this gene phosphorylates vitamin B6, a step required for the conversion of vitamin B6 to pyridoxal-5-phosphate, an important cofactor in intermediary metabolism. The encoded protein is cytoplasmic and probably acts as a homodimer. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

PDXK: Required for synthesis of pyridoxal-5-phosphate from vitamin B6. Belongs to the pyridoxine kinase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cofactor and Vitamin Metabolism - vitamin B6; Kinase, other; EC 2.7.1.35

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: cytoplasm; cytosol; nucleoplasm; nucleus

Molecular Function: ATP binding; lithium ion binding; magnesium ion binding; potassium ion binding; protein homodimerization activity; pyridoxal kinase activity; pyridoxal phosphate binding; sodium ion binding; zinc ion binding

Biological Process: cell proliferation; pyridoxal 5'-phosphate salvage; pyridoxal phosphate biosynthetic process; vitamin B6 metabolic process

Research Articles on PDXK

Similar Products

Product Notes

The PDXK pdxk (Catalog #AAA7043630) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PDXK Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PDXK can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). WB:1:1000-1:5000 IHC:1:20-1:200. Researchers should empirically determine the suitability of the PDXK pdxk for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PDXK, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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