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Testing Data (Optimal Staining of PP2C using MBS6004030 in human Testis)

Rabbit anti-Human, Rat PDP1 Polyclonal Antibody | anti-PPAPDC2 antibody

PDP1 (Pyruvate Dehyrogenase Phosphatase Catalytic Subunit 1, PDH, PDP, PDP 1, PDPC, PDPC 1, PPM2C, Protein phosphatase 2C)

Gene Names
PPAPDC2; PDP1; PSDP; bA6J24.6
Reactivity
Human, Rat
Applications
Western Blot
Purity
Purified
Purified by ammonium sulfate precipitation.
Synonyms
PDP1; Polyclonal Antibody; PDP1 (Pyruvate Dehyrogenase Phosphatase Catalytic Subunit 1; PDH; PDP; PDP 1; PDPC; PDPC 1; PPM2C; Protein phosphatase 2C); Anti -PDP1 (Pyruvate Dehyrogenase Phosphatase Catalytic Subunit 1; anti-PPAPDC2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human Protein Phosphatase 2 C alpha/beta. Species crossreactivity
Purity/Purification
Purified
Purified by ammonium sulfate precipitation.
Form/Format
Supplied as a liquid in PBS with 0.08% sodium azide.
Applicable Applications for anti-PPAPDC2 antibody
Western Blot (WB)
Application Notes
Suitable for use in Western Blot.
Dilution: Western Blot: 1:500
Immunogen
Synthetic peptide corresponding to human Protein Phosphatase 2 C alpha/beta (KLH).
Positive Control
L6 Cell Lysate.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Testing Data

(Optimal Staining of PP2C using MBS6004030 in human Testis)

Testing Data (Optimal Staining of PP2C using MBS6004030 in human Testis)

Immunohistochemistry (IHC)

(PP2C staining in normal human testis, tissue pretreated with citrate buffer, pH 6.0. MBS6004030 was diluted to 1:100, using IHC-Select® detection with HRP-DAB.)

Immunohistochemistry (IHC) (PP2C staining in normal human testis, tissue pretreated with citrate buffer, pH 6.0. MBS6004030 was diluted to 1:100, using IHC-Select® detection with HRP-DAB.)

Western Blot (WB)

(Western Blot Analysis: L6 lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with MBS6004030 (1:500 dilution of this lot). Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates protein PP2C ALPHA/BETA (~46kD).)

Western Blot (WB) (Western Blot Analysis: L6 lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with MBS6004030 (1:500 dilution of this lot). Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates protein PP2C ALPHA/BETA (~46kD).)

Testing Data

(Huvec lysate (Lane 1) and L6 lysate (Lane 2) were resolved by electrophoresis, transferred to PVDF membrane and probed with MBS6004030 (1:500 dilution of a previous lot). Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates protein PP2C ALPHA/BETA (~46kD).)

Testing Data (Huvec lysate (Lane 1) and L6 lysate (Lane 2) were resolved by electrophoresis, transferred to PVDF membrane and probed with MBS6004030 (1:500 dilution of a previous lot). Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates protein PP2C ALPHA/BETA (~46kD).)
Related Product Information for anti-PPAPDC2 antibody
PP-2 C alpha/beta is heterodimer located in the mitochondrial matrix. Within the pyruvate dehydrogenase complex, PP-2 C alpha/beta serves to catalyze the dephosphorylation and concomitant reactivation of the E1 component's alpha subunit. Mutations occurring in the PDP1 gene are the cause of a disease known as pyruvate dehydrogenase deficiency (PDP deficiency), which leads to neurological dysfunction from lactic acidosis.
Product Categories/Family for anti-PPAPDC2 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
32,194 Da
NCBI Official Full Name
presqualene diphosphate phosphatase
NCBI Official Synonym Full Names
phosphatidic acid phosphatase type 2 domain containing 2
NCBI Official Symbol
PPAPDC2
NCBI Official Synonym Symbols
PDP1; PSDP; bA6J24.6
NCBI Protein Information
presqualene diphosphate phosphatase; PPAP2 domain-containing protein 2; polyisoprenoid diphosphate phosphatase type 1; phosphatidic acid phosphatase type 2 domain-containing protein 2
UniProt Protein Name
Presqualene diphosphate phosphatase
UniProt Gene Name
PPAPDC2
UniProt Synonym Gene Names
PPAP2 domain-containing protein 2
UniProt Entry Name
PPAC2_HUMAN

Uniprot Description

PPAPDC2: Phosphatase that dephosphorylates presqualene diphosphate (PSDP) into presqualene monophosphate (PSMP), suggesting that it may be indirectly involved in innate immunity. PSDP is a bioactive lipid that rapidly remodels to presqualene monophosphate PSMP upon cell activation. Displays diphosphate phosphatase activity with a substrate preference for PSDP > FDP > phosphatidic acid. Belongs to the PA-phosphatase related phosphoesterase family.

Protein type: Membrane protein, integral; Phosphatase; Membrane protein, multi-pass; EC 3.1.3.-

Chromosomal Location of Human Ortholog: 9p24.1

Cellular Component: integral to membrane

Molecular Function: hydrolase activity

Biological Process: metabolic process

Research Articles on PPAPDC2

Similar Products

Product Notes

The PPAPDC2 ppapdc2 (Catalog #AAA6004030) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PDP1 (Pyruvate Dehyrogenase Phosphatase Catalytic Subunit 1, PDH, PDP, PDP 1, PDPC, PDPC 1, PPM2C, Protein phosphatase 2C) reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PDP1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot. Dilution: Western Blot: 1:500. Researchers should empirically determine the suitability of the PPAPDC2 ppapdc2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PDP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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