Rabbit PDLIM4 Polyclonal Antibody | anti-PDLIM4 antibody

PDLIM4 Antibody

Cat. Num. AAA9631288

• Gene Names: PDLIM4; RIL
• Reactivity: Human, Mouse, Rat
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: PDLIM4; Polyclonal Antibody; PDLIM4 Antibody; Enigma homolog; LIM domain protein; LIM domain protein RIL; LIM protein RIL; PDLI4_HUMAN; PDLIM 4; PDZ and LIM domain 4; PDZ and LIM domain protein 4; Reversion induced LIM protein; Reversion-induced LIM protein; anti-PDLIM4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: PDLIM4 Antibody detects endogenous levels of PDLIM4.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-PDLIM4 antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human PDLIM4.
• Conjugation: Unconjugated
• Post Translational Modifications: Phosphorylated on tyrosine residue(s). Can be dephosphorylated by PTPN13.
• Subunit Structure: Homodimer. Interacts with PTPN13. Interacts (via C-terminus only or via combined C-terminus and LIM domain, but not LIM domain only) with PTPN13 (via the second or fourth PDZ domains). Found in a complex with PTPN13 and TRIP6 (By similarity). Interacts (via PDZ domain) with ACTN1 and ACTN2 (via C-terminal SDL residues) (By similarity). Interacts (via PDZ domain) with TRIP6 (via the second LIM domain or via the third LIM domain plus C-terminus). Interacts (via LIM domain) with GRIA1 (via C-terminus); this interaction as well as the interaction with alpha-actinin is required for their colocalization in early endosomes. Interacts with PDLIM1 (By similarity). Forms (via LIM domain) a heterodimer with PDLIM3 (By similarity). Interacts directly with SRC (via kinase domain and to a lesser extent the SH2 domain). Isoform 2 interacts with NQO1. NQO1-stabilized isoform 2 heterodimerizes with isoform 1.
• Tissue Specificity: Isoform 2 is found in brain.
• Function: Suppresses SRC activation by recognizing and binding to active SRC and facilitating PTPN13-mediated dephosphorylation of SRC 'Tyr-419' leading to its inactivation. Inactivated SRC dissociates from this protein allowing the initiation of a new SRC inactivation cycle. Involved in reorganization of the actin cytoskeleton. In nonmuscle cells, binds to ACTN1 (alpha-actinin-1), increases the affinity of ACTN1 to F-actin (filamentous actin), and promotes formation of actin stress fibers. Involved in regulation of the synaptic AMPA receptor transport in dendritic spines of hippocampal pyramidal neurons directing the receptors toward an insertion at the postsynaptic membrane. Links endosomal surface-internalized GRIA1-containing AMPA receptors to the alpha-actinin/actin cytoskeleton. Increases AMPA receptor-mediated excitatory postsynaptic currents in neurons (By similarity).Involved in reorganization of the actin cytoskeleton and in regulation of cell migration. In response to oxidative stress, binds to NQO1, which stabilizes it and protects it from ubiquitin-independent degradation by the core 20S proteasome. Stabilized protein is able to heterodimerize with isoform 1 changing the subcellular location of it from cytoskeleton and nuclei to cytosol, leading to loss of isoforms 1 ability to induce formation of actin stress fibers. Counteracts the effects produced by isoform 1 on organization of actin cytoskeleton and cell motility to fine-tune actin cytoskeleton rearrangement and to attenuate cell migration.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-PDLIM4 antibody

Cytoplasm; Cytoskeleton; Nucleus; Perinuclear region; Cell projection; Lamellipodium; Dendritic spine; Early endosome membrane; Peripheral membrane protein; Recycling endosome membrane; Cell junction; Synapse; Synaptosome; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 19923181
• NCBI GeneID: 8572
• NCBI Accession #: NP_003678.2
• NCBI GenBank Nucleotide #: NM_003687.3
• UniProt Accession #: P50479; Q53Y39; Q96AT8; Q9BTW8; Q9Y292; B2R8U1
• Molecular Weight: 25,854 Da
• NCBI Official Full Name: PDZ and LIM domain protein 4 isoform 1
• NCBI Official Synonym Full Names: PDZ and LIM domain 4
• NCBI Official Symbol: PDLIM4
• NCBI Official Synonym Symbols: RIL
• NCBI Protein Information: PDZ and LIM domain protein 4; enigma homolog; LIM protein RIL; reversion-induced LIM protein
• UniProt Protein Name: PDZ and LIM domain protein 4
• Protein Family: PDZ and LIM domain protein
• UniProt Gene Name: PDLIM4
• UniProt Synonym Gene Names: RIL
• UniProt Entry Name: PDLI4_HUMAN

NCBI Description

This gene encodes a protein which may be involved in bone development. Mutations in this gene are associated with susceptibility to osteoporosis. [provided by RefSeq, Nov 2009]

Uniprot Description

RIL: 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 5q31.1

Molecular Function: protein binding; zinc ion binding

Disease: Osteoporosis