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Immunohistochemistry (IHC) (Anti-PDLIM4 / RIL antibody IHC staining of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Goat PDLIM4 / RIL Polyclonal Antibody | anti-PDLIM4 antibody

Goat Polyclonal to Human PDLIM4 / RIL

Gene Names
PDLIM4; RIL
Reactivity
Bat, Gibbon, Bovine, Chicken, Dog, Xenopus, Gorilla, Hamster, Horse, Human, Monkey, Mouse, Pig, Rabbit, Rat
Predicted Reactivity: Zebrafish (at least 90% immunogen sequence identity)
Applications
Immunohistochemistry, Western Blot, ELISA
Purity
Immunoaffinity Purified
Synonyms
PDLIM4 / RIL; Polyclonal Antibody; Goat Polyclonal to Human PDLIM4 / RIL; Anti-PDLIM4 / RIL Antibody (C-Terminus) IHC-plus; PDLIM4; LIM protein RIL; LIM domain protein; Reversion-induced LIM protein; PDZ and LIM domain 4; Enigma homolog; PDZ and LIM domain protein 4; RIL; Human PDLIM4; anti-PDLIM4 antibody
Ordering
For Research Use Only!
Host
Goat
Reactivity
Bat, Gibbon, Bovine, Chicken, Dog, Xenopus, Gorilla, Hamster, Horse, Human, Monkey, Mouse, Pig, Rabbit, Rat
Predicted Reactivity: Zebrafish (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Specificity
Human PDLIM4.
Purity/Purification
Immunoaffinity Purified
Form/Format
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Concentration
0.5 mg/ml (varies by lot)
Sequence Length
330
Applicable Applications for anti-PDLIM4 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes
ELISA (1:32000), IHC-P (3.75 ug/ml), WB (0.1 - 0.5 ug/ml)
Target Species
Human
Immunogen Description
Synthetic peptide C-DVVAVYPNAKVELV from the C-terminus of human PDLIM4 (NP_003678.2).
Immunogen Type
Synthetic peptide
Immunogen
PDLIM4 / RIL antibody was raised against synthetic peptide C-DVVAVYPNAKVELV from the C-terminus of human PDLIM4 (NP_003678.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Shrew, Hamster, Elephant, Dog, Bat, Bovine, Rabbit, Horse, Pig, Opossum, Turkey, Chicken, Armadillo, Xenopus (100%); Galago, Salmon, Stickleback, Zebrafish (93%); Pufferfish (86%).
Antigen Modification
C-Terminus
Preparation and Storage
Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-PDLIM4 / RIL antibody IHC staining of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Immunohistochemistry (IHC) (Anti-PDLIM4 / RIL antibody IHC staining of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Western Blot (WB)

(Antibody staining (0.2 ug/ml) of A431 lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)

Western Blot (WB) (Antibody staining (0.2 ug/ml) of A431 lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
25,854 Da
NCBI Official Full Name
PDZ and LIM domain protein 4 isoform 1
NCBI Official Synonym Full Names
PDZ and LIM domain 4
NCBI Official Symbol
PDLIM4
NCBI Official Synonym Symbols
RIL
NCBI Protein Information
PDZ and LIM domain protein 4; enigma homolog; LIM protein RIL; reversion-induced LIM protein
UniProt Protein Name
PDZ and LIM domain protein 4
UniProt Gene Name
PDLIM4
UniProt Synonym Gene Names
RIL
UniProt Entry Name
PDLI4_HUMAN

NCBI Description

This gene encodes a protein which may be involved in bone development. Mutations in this gene are associated with susceptibility to osteoporosis. [provided by RefSeq, Nov 2009]

Uniprot Description

RIL: 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 5q31.1

Molecular Function: protein binding; zinc ion binding

Disease: Osteoporosis

Research Articles on PDLIM4

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Product Notes

The PDLIM4 pdlim4 (Catalog #AAA248783) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat Polyclonal to Human PDLIM4 / RIL reacts with Bat, Gibbon, Bovine, Chicken, Dog, Xenopus, Gorilla, Hamster, Horse, Human, Monkey, Mouse, Pig, Rabbit, Rat Predicted Reactivity: Zebrafish (at least 90% immunogen sequence identity) and may cross-react with other species as described in the data sheet. AAA Biotech's PDLIM4 / RIL can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA). ELISA (1:32000), IHC-P (3.75 ug/ml), WB (0.1 - 0.5 ug/ml). Researchers should empirically determine the suitability of the PDLIM4 pdlim4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PDLIM4 / RIL, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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