Rabbit anti-Human, Mouse Pdk2 Polyclonal Antibody | anti-Pdk2 antibody

Pdk2, NT (Pdk2, [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 2, mitochondrial, Pyruvate dehydrogenase kinase isoform 2) (MaxLight 550)

Cat. Num. AAA6332426

• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Pdk2; Polyclonal Antibody; NT (Pdk2; [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 2; mitochondrial; Pyruvate dehydrogenase kinase isoform 2) (MaxLight 550); Pyruvate dehydrogenase kinase isoform 2; anti-Pdk2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight550.

• Applicable Applications for anti-Pdk2 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: Mouse Pdk2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 77-107 amino acids from the N-terminal region of Mouse Pdk2.
• Conjugate: MaxLight550
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Related Product Information for anti-Pdk2 antibody

Pdk2 inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.

Product Categories/Family for anti-Pdk2 antibody

Antibodies; Protein Kinases

References

Sun, W., et al. Clin. Cancer Res. 15(2):476-484(2009). Pagliarini, D.J., et al. Cell 134(1):112-123(2008). Osafune, K., et al. Development 133(1):151-161(2006). Papin, J., et al. Curr. Opin. Biotechnol. 15(1):78-81(2004). Mootha, V.K., et al. Cell 115(5):629-640(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 226958643
• NCBI GeneID: 18604
• NCBI Accession #: NP_598428.2
• NCBI GenBank Nucleotide #: NM_133667.2
• UniProt Accession #: Q9JK42; Q8VC63
• Molecular Weight: 46,041 Da
• NCBI Official Full Name: pyruvate dehydrogenase kinase, isoenzyme 2
• NCBI Official Synonym Full Names: pyruvate dehydrogenase kinase, isoenzyme 2
• NCBI Official Symbol: Pdk2
• NCBI Protein Information: pyruvate dehydrogenase kinase, isoenzyme 2
• UniProt Protein Name: [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 2, mitochondrial
• UniProt Gene Name: Pdk2
• UniProt Synonym Gene Names: PDH kinase 2
• UniProt Entry Name: PDK2_MOUSE

Uniprot Description

PDHK2: an atypical protein kinase associated with the mitochondrial matrix. The PDHKs play crucial roles in switching metabolic flux from oxidative phosphorylation towards glycolysis. PDHK2 is expressed in all tissues. Contains a HATPase_c catalytic domain, found in several ATP-binding proteins including protein histidine kinases (PHKs), PHDKs, DNA gyrase B, topoisomerases, heat shock proteins, and DNA mismatch repair proteins. PDHK regulates glucose oxidation through inhibitory phosphorylation of the E1 alpha subunit of the mitochondrial pyruvate dehydrogenase complex (PDHC) at any one of 3 inhibitory serine residues. Inhibitory sites 1, 2, and 3 correspond to S293, S300, and S232 in human PDHA1, respectively. Four PDHK isoenzymes have been described, each with different site specificity: all four phosphorylate sites 1 and 2 but at different rates; for site 1 PDHK2 >PDHK4 >PDHK1 >PDHK3; for site 2, PDHK3> PDHK4 > PDHK2 > PDHK1. Only PDHK1 phosphorylates site 3. PDHKs are recruited to the PDHC by binding to a lipoyl group covalently attached to the inner lipoyl domain of the E2 component. PDHA1 deficiency is the most common enzyme defect in patients with primary lactic acidosis. Suppression of PDH by PDHK inhibits the conversion of pyruvate to acetyl-CoA, attenuates mitochondrial respiration, and may contribute to the increased lactate production observed in many tumors. The PDH pathway is repressed in a majority of non-small cell lung carcinomas. Inhibited by AZD7545, dichloroacetate (DCA), and radicicol. Radicicol inhibits kinase activity by binding directly to the ATP-binding pocket of PDHK, similar to HSP90 from the same ATPase/kinase superfamily.

Protein type: EC 2.7.11.2; Protein kinase, atypical; Kinase, protein; Mitochondrial; ATYPICAL group; PDHK family; PDHK subfamily

Cellular Component: nucleoplasm; mitochondrion; cytoplasm; pyruvate dehydrogenase complex; mitochondrial pyruvate dehydrogenase complex

Molecular Function: transferase activity; protein serine/threonine kinase activity; protein binding; protein homodimerization activity; protein heterodimerization activity; nucleotide binding; protein complex binding; kinase activity; pyruvate dehydrogenase (acetyl-transferring) kinase activity; ATP binding

Biological Process: peptidyl-serine phosphorylation; carbohydrate metabolic process; insulin receptor signaling pathway; glucose metabolic process; regulation of pH; cellular response to nutrient; regulation of acetyl-CoA biosynthetic process from pyruvate; regulation of gluconeogenesis; glucose homeostasis; phosphorylation; protein amino acid phosphorylation