CEACAM6 elisa pair set

CEACAM6 Matched ELISA Antibody Pair Set, Human

Cat. Num. AAA8118307

• Synonyms: CEACAM6; CEACAM6 Matched ELISA Antibody Pair Set; Human; Human CEACAM6/CD66c ELISA Pair Set; carcinoembryonic antigen-related cell adhesion molecule 6 (non-specific cross reacting antigen); CD66c Matched ELISA Antibody Pair Set; CEA Matched ELISA Antibody Pair Set; CEAL Matched ELISA Antibody Pair Set; NCA Matched ELISA Antibody Pair Set; CEACAM6 elisa pair set

• Specificity: Quantitative determination of Human CEACAM6

• Assay Type: Solid Phase Sandwich ELISA
• Detection Range: 15.6-1000pg/mL
• Sensitivity: 15.6pg/ml
• Materials Provided: 1. Capture Antibody 1 mg/mL of mouse anti-CEACAM6 monoclonal antibody. Dilute to a working concentration of 2ug/mL in PBS before coating.2. Detection Antibody 0.4 mg/mL of biotinylated rabbit anti-CEACAM6 polyclonal antibody. Dilute to working concentration of 0.5ug/mL in detection antibody dilution buffer before use.3. Standard Each vial contains 88 ng of recombinant CEACAM6. Reconstitute standard powder with 1 mL detection antibody dilution buffer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 1000 pg/mL is recommended.4. Streptavidin-HRP 50uL of streptavidin conjugated to horseradish-peroxidase. 1:2000 Dilution in detection antibody dilution buffer before use.
• Preparation and Storage: Capture Antibody: Aliquot and store at -20 degree C to -80 degree C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.; Detection Antibody: Aliquot and store at -20 degree C to -80 degree C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.; Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.Streptavidin-HRP: Store at 4 degree C and protect it from prolonged exposure to light. DO NOT FREEZE! It is stable for up to 6 months from date of receipt.

Testing Data

Related Product Information for CEACAM6 elisa pair set

Carcinoembryonic antigen-related cell adhesion molecule 6 (CEACAM6), also known as nonspecific crossreacting antigen (NCA) and CD66c, is one of seven human CEACAM family members within the immunoglobulin superfamily. It s a glycosylphosphatidylinositol-linked immunoglobulin superfamily member that is overexpressed in a variety of human cancers, including colon, breast and lung and is associated with tumourigenesis, tumour cell adhesion, invasion and metastasis. CEACAM6 is a unique mediator of migration and invasion of drug resistant oestrogen-deprived breast cancer cells, and this protein could be an important biomarker of metastasis. CEACAM6 is expressed by granulocytes and their progenitors. It is also expressed by epithelia of various organs and is upregulated in pancreatic and colon adenocarcinomas, as well as hyperplastic polyps. Resistance to adhesion-related apoptosis in tumor cells is conferred in the condition of CEACAM6 overexpression.

This CEACAM6 Matched ELISA Antibody Pair Set, Human is a solid phase sandwich ELISA for quantitative determination of Human CEACAM6. It contains Human CEACAM6 capture antibody, Human CEACAM6 detector antibody (Biotin) Streptavidin-HRP and a highly purified HEK293-expressed recombinant Human CEACAM6 protein.

References

Duxbury MS, et al. (2004) Overexpression of CEACAM6 promotes insulin-like growth factor I-induced pancreatic adenocarcinoma cellular invasiveness. Oncogene. 23(34): 5834-42.Lewis-Wambi JS, et al. (2008) Overexpression of CEACAM6 promotes migration and invasion of oestrogen-deprived breast cancer cells. Eur J Cancer. 44(12): 1770-9.