Western Blot (WB)
(Staining (1ug/ml) of HepG2 lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
Goat PDIA3/ERp57/GRP58 Polyclonal Antibody | anti-PDIA3 antibody
Goat anti-PDIA3/ERp57/GRP58 Antibody
Western Blot: Approx 55kDa band observed in lysates of cell lines HepG2 and NIH3T3 (calculated MW of 56.8kDa according to NP_005304.3). Recommended concentration: 1-3ug/ml.
Western Blot (WB)
(Staining (1ug/ml) of HepG2 lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
Western Blot (WB)
(Staining (1ug/ml) of HepG2 lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a protein of the endoplasmic reticulum that interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. The protein was once thought to be a phospholipase; however, it has been demonstrated that the protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and this protein mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates. [provided by RefSeq, Jul 2008]
Uniprot Description
GRP58: a protein disulfide isomerase found in the endoplasmic reticulum lumen. Catalyzes the rearrangement of both intrachain and interchain disulfide bonds in proteins to form the native structures. An essential component of the peptide-loading complex of the major histocompatibility complex class I pathway.
Protein type: EC 5.3.4.1; Chaperone; Apoptosis; Protease; Isomerase; Endoplasmic reticulum; Phospholipase
Chromosomal Location of Human Ortholog: 15q15
Cellular Component: cell surface; endoplasmic reticulum; endoplasmic reticulum lumen; focal adhesion; melanosome; myelin sheath; nucleus
Molecular Function: cysteine-type endopeptidase activity; disulfide oxidoreductase activity; phospholipase C activity; protein binding; protein disulfide isomerase activity
Biological Process: antigen processing and presentation of peptide antigen via MHC class I; cell redox homeostasis; protein folding; protein import into nucleus; protein retention in ER; proteolysis; signal transduction
Research Articles on PDIA3
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Product Notes
The PDIA3 pdia3 (Catalog #AAA421355) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-PDIA3/ERp57/GRP58 Antibody reacts with Tested: Human, Mouse; Expected from sequence similarity: Human, Mouse, Rat, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's PDIA3/ERp57/GRP58 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 8000. Western Blot: Approx 55kDa band observed in lysates of cell lines HepG2 and NIH3T3 (calculated MW of 56.8kDa according to NP_005304.3). Recommended concentration: 1-3ug/ml. Researchers should empirically determine the suitability of the PDIA3 pdia3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: EEKPKKKKKA QEDL. It is sometimes possible for the material contained within the vial of "PDIA3/ERp57/GRP58, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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