Western Blot (WB)
(Biotinylated (0.03ug/ml) staining of Rat Pancreas lysate (35ug protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.)
Goat PDIA2 Polyclonal Antibody | anti-PDIA2 antibody
Goat Anti-PDIA2 (aa477-481), Biotinylated Antibody
Gene Names
PDIA2; PDI; PDA2; PDIP; PDIR
Reactivity
Human, Mouse, RatTested: Human, Mouse, Rat
Expected from sequence similarity: Human, Mouse, Rat
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
PDIA2; Polyclonal Antibody; Goat Anti-PDIA2 (aa477-481); Biotinylated Antibody; Goat Anti-PDIA2 / PDIP Antibody; PDIA2 antibody; protein disulfide isomerase family A member 2 antibody; PDA2 antibody; PDI antibody; PDIP antibody; PDIR antibody; Rho GDP dissociation inhibitor gamma antibody; pancreas-specific protein disulfide isomerase antibody; pancreatic protein disulfide isomerase antibody; protein disulfide isomerase A2 antibody; protein disulfide isomerase family A; member 2 antibody; protein disulfide isomerase; pancreatic antibody; protein disulfide isomerase-associated 2 antibody; anti-PDIA2 antibody
Host
Goat
Reactivity
Human, Mouse, Rat
Tested: Human, Mouse, Rat
Expected from sequence similarity: Human, Mouse, Rat
Tested: Human, Mouse, Rat
Expected from sequence similarity: Human, Mouse, Rat
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Sequence
EYKSTRDLETFSK.
Sequence Length
525
Applicable Applications for anti-PDIA2 antibody
Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Western Blot: Approx 65kDa band observed in Rat Pancreas lysates (calculated MW of 58.5kDa according to Rat NP_001099245.2). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration: 0.03-0.1ug/ml.
Peptide ELISA: Antibody detection limit dilution 1:64000.
Peptide ELISA: Antibody detection limit dilution 1:64000.
Immunogen
Peptide with sequence C-EYKSTRDLETFSK., from the internal region (near C Terminus) of the protein sequence according to NP_006840.2.
Epitope
Internal Region (near C Terminus)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Western Blot (WB)
(Biotinylated (0.03ug/ml) staining of Rat Pancreas lysate (35ug protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.)
Western Blot (WB)
(Biotinylated (0.03ug/ml) staining of Rat Pancreas lysate (35ug protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
57,850 Da
NCBI Official Full Name
protein disulfide-isomerase A2
NCBI Official Synonym Full Names
protein disulfide isomerase family A member 2
NCBI Official Symbol
PDIA2
NCBI Official Synonym Symbols
PDI; PDA2; PDIP; PDIR
NCBI Protein Information
protein disulfide-isomerase A2
UniProt Protein Name
Protein disulfide-isomerase A2
Protein Family
UniProt Gene Name
PDIA2
UniProt Synonym Gene Names
PDIP; PDIp
UniProt Entry Name
PDIA2_HUMAN
NCBI Description
Protein disulfide isomerases (EC 5.3.4.1), such as PDIP, are endoplasmic reticulum (ER) resident proteins that catalyze protein folding and thiol-disulfide interchange reactions (Desilva et al., 1996 [PubMed 8561901]).[supplied by OMIM, Mar 2008]
Uniprot Description
PDIA2: Acts as an intracellular estrogen-binding protein. May be involved in modulating cellular levels and biological functions of estrogens in the pancreas. May act as a chaperone that inhibits aggregation of misfolded proteins. Belongs to the protein disulfide isomerase family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Endoplasmic reticulum; Isomerase; EC 5.3.4.1
Chromosomal Location of Human Ortholog: 16p13.3
Cellular Component: endoplasmic reticulum
Molecular Function: disulfide oxidoreductase activity; peptidyl-proline 4-dioxygenase activity; protein binding
Biological Process: protein folding; protein retention in ER
Research Articles on PDIA2
Similar Products
Product Notes
The PDIA2 pdia2 (Catalog #AAA423689) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat Anti-PDIA2 (aa477-481), Biotinylated Antibody reacts with Human, Mouse, Rat Tested: Human, Mouse, Rat Expected from sequence similarity: Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PDIA2 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Western Blot: Approx 65kDa band observed in Rat Pancreas lysates (calculated MW of 58.5kDa according to Rat NP_001099245.2). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration: 0.03-0.1ug/ml. Peptide ELISA: Antibody detection limit dilution 1:64000. Researchers should empirically determine the suitability of the PDIA2 pdia2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: EYKSTRDLET FSK. It is sometimes possible for the material contained within the vial of "PDIA2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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