Rabbit PDGFB Polyclonal Antibody | anti-PDGFB antibody

PDGFB Antibody

Cat. Num. AAA9404630

• Gene Names: PDGFB; SIS; SSV; IBGC5; PDGF2; c-sis; PDGF-2
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Antibodies were purified by affinity purification using immunogen.
• Synonyms: PDGFB; Polyclonal Antibody; PDGFB Antibody; FLJ12858; PDGF2; SIS; SSV; anti-PDGFB antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total PDGFB protein.
• Purity/Purification: Antibodies were purified by affinity purification using immunogen.
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 241

• Applicable Applications for anti-PDGFB antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Western blotting: 1:500 - 1:2000; Immunohistochemistry: 1:50 - 1:100
• Immunogen Type: Recombinant Protein
• Immunogen Description: Recombinant protein of human PDGFB.
• Target Name: PDGFB
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(Western blot analysis of extracts of A431 cell lines using PDGFB antibody.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human stomach cancer using PDGFB antibody at dilution of 1:200 (400x lens).)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded rat spleen using PDGFB antibody at dilution of 1:100 (400x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded humanesophagus using PDGFB at dilution of 1:100 (40x lens).)

Related Product Information for anti-PDGFB antibody

PDGF-BB is a cystine-linked homodimer PDGF family member with key roles in development, cell proliferation, cell survival, and angiogenesis (1,2). PDGF-BB is expressed by vascular endothelium, megakaryocytes and Leydig cells (2). PDGF-BB targets pericytes, fibroblasts, monocytes and other cell types (1-3). PDGF induces fibroblast growth and migration (3) and is a chemoattractant for monocytes and granulocytes. Precursor PDGF-BB is cleaved intracellularly to generate a form that contains a carboxy-terminal stretch that serves to retain PDGF-BB in the extracellular matrix. In a second cleavage event, the carboxy-terminal stretch is removed extracellularly to generate mature PDGF-BB (1,2). PDGF-BB binds to PDGFRb and induces receptor dimerization. Signaling is through the PI3K/Akt, JNK, and PLCg pathways (1, 2). PDGF-BB may have a role in some cancer types (2).

Product Categories/Family for anti-PDGFB antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 4505681
• NCBI GeneID: 5155
• NCBI Accession #: NP_002599.1
• NCBI GenBank Nucleotide #: NM_002608.2
• UniProt Accession #: P01127; P78431; Q15354; Q6FHE7; Q9UF23; G3XAG8
• Molecular Weight: 27KD
• NCBI Official Full Name: platelet-derived growth factor subunit B isoform 1 preproprotein
• NCBI Official Synonym Full Names: platelet-derived growth factor beta polypeptide
• NCBI Official Symbol: PDGFB
• NCBI Official Synonym Symbols: SIS; SSV; IBGC5; PDGF2; c-sis; PDGF-2
• NCBI Protein Information: platelet-derived growth factor subunit B
• UniProt Protein Name: Platelet-derived growth factor subunit B
• Protein Family: Platelet-derived growth factor
• UniProt Gene Name: PDGFB
• UniProt Synonym Gene Names: PDGF2; SIS; PDGF subunit B
• UniProt Entry Name: PDGFB_HUMAN

NCBI Description

The protein encoded by this gene is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. This gene product can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds. Mutations in this gene are associated with meningioma. Reciprocal translocations between chromosomes 22 and 17, at sites where this gene and that for collagen type 1, alpha 1 are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans resulting from unregulated expression of growth factor. Two alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Feb 2013]

Uniprot Description

Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin (PubMed:26599395). Required for normal proliferation and recruitment of pericytes and vascular smooth muscle cells in the central nervous system, skin, lung, heart and placenta. Required for normal blood vessel development, and for normal development of kidney glomeruli. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFA ().