PDGF-BB active protein

Human PDGF-BB

Cat. Num. AAA692013

• Gene Names: PDGFB; SIS; SSV; IBGC5; PDGF2; c-sis; PDGF-2
• Reactivity: Human
• Purity: > 95% by SDS-PAGE & silver stain
• Synonyms: PDGF-BB; Human PDGF-BB; Recombinant Human Platelet-Derived Growth Factor-BB; Platelet-derived growth factor B chain; PDGF2; Proto-oncogene c-Sis; PDGF-BB active protein

• Host: E Coli
• Reactivity: Human
• Purity/Purification: > 95% by SDS-PAGE & silver stain
• Form/Format: Lyophilized
• Sequence: N-Terminal Sequence: UNDER WORK!
• Sequence Length: 109

• Endotoxin Level: < 0.1ng per ug of PDGF-BB
• Buffer: 50 mM acetic acid
• Preparation and Storage: The lyophilized PDGF-BB is stable for a few weeks at room temperature, but best stored at -20 degree C. Reconstituted PDGF-BB is best stored at -20 degree C to -70 degree C.

Testing Data

Testing Data #2

Related Product Information for PDGF-BB active protein

PDGFs are disulfide-linked dimers consisting of two 12.0-13.5 kDa polypeptide chains, designated PDGF-A and PDGF-B chains. The three naturally occurring PDGFs; PDGF-AA, PDGF-BB and PDGF-AB, are potent mitogens for a variety of cell types including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. The PDGFs are stored in platelet alpha-granules and are released upon platelet activation. The PDGFs are involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. Two distinct signaling receptors used by PDGFs have been identified and named PDGFR-alpha and PDGFR-beta. PDGFR-alpha is high-affinity receptor for each of the three PDGF forms. On the other hand, PDGFR-beta interacts with only PDGF-BB and PDGF-AB. Recombinant human PDGF-BB is a 24.3 kDa disulfide-linked homodimer of two B chains (218 total amino acids).

Product Categories/Family for PDGF-BB active protein

Cytokines & Growth Factors

References

1. Fredriksson L et al, Cytokine Growth Factor Rev 15:197-204, 2004 2. Heldin CH et al, Br J Cancer 57:591-3, 1988 3. Deuel TFet al, Biofactors 1:213-7, 1988 4. Meyer-Ingold and Eichner W, Cell Biol Int 19:389-98, 1995 5. Betsholtz C and Raines EW, Kidney Int 51:1361-9, 1997 6. Kaetzel DM, Cytokine Growth Factor Rev 14:427-46, 2003 7. Simm A et al, Basic Res Cardiol Suppl 3:40-3, 1998

NCBI and Uniprot Product Information

• NCBI GI #: 4505681
• NCBI GeneID: 5155
• NCBI Accession #: NP_002599.1
• NCBI GenBank Nucleotide #: NM_002608.2
• UniProt Accession #: P01127; P78431; Q15354; Q6FHE7; Q9UF23; G3XAG8
• Molecular Weight: 24,3 kDa
• NCBI Official Full Name: platelet-derived growth factor subunit B isoform 1 preproprotein
• NCBI Official Synonym Full Names: platelet-derived growth factor beta polypeptide
• NCBI Official Symbol: PDGFB
• NCBI Official Synonym Symbols: SIS; SSV; IBGC5; PDGF2; c-sis; PDGF-2
• NCBI Protein Information: platelet-derived growth factor subunit B; becaplermin; PDGF, B chain; PDGF subunit B; proto-oncogene c-Sis; platelet-derived growth factor 2; platelet-derived growth factor B chain; platelet-derived growth factor, beta polypeptide (oncogene SIS); platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog)
• UniProt Protein Name: Platelet-derived growth factor subunit B
• UniProt Gene Name: PDGFB
• UniProt Synonym Gene Names: PDGF2; SIS; PDGF subunit B
• UniProt Entry Name: PDGFB_HUMAN

NCBI Description

The protein encoded by this gene is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. This gene product can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds. Mutations in this gene are associated with meningioma. Reciprocal translocations between chromosomes 22 and 17, at sites where this gene and that for collagen type 1, alpha 1 are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans resulting from unregulated expression of growth factor. Two alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Feb 2013]

Uniprot Description

Function: Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal proliferation and recruitment of pericytes and vascular smooth muscle cells in the central nervous system, skin, lung, heart and placenta. Required for normal blood vessel development, and for normal development of kidney glomeruli. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFA

By similarity.

Subunit structure: Homodimer; antiparallel disulfide-linked dimer. Heterodimer with PDGFA; antiparallel disulfide-linked dimer. The PDGFB homodimer interacts with PDGFRA and PDGFRB homodimers, and with heterodimers formed by PDGFRA and PDGFRB. The heterodimer composed of PDGFA and PDGFB interacts with PDGFRB homodimers, and with heterodimers formed by PDGFRA and PDGFRB. Interacts with XLKD1

By similarity. Ref.19 Ref.24

Subcellular location: Secreted. Note: Released by platelets upon wounding.

Tissue specificity: Expressed at high levels in the heart, brain (sustantia nigra), placenta and fetal kidney. Expressed at moderate levels in the brain (hippocampus), skeletal muscle, kidney and lung. Ref.20

Involvement in disease: A chromosomal aberration involving PDGFB is found in dermatofibrosarcoma protuberans. Translocation t(17;22)(q22;q13) with PDGFB. Ref.21

Pharmaceutical use: Available under the name Regranex (Ortho-McNeil). Used to promote healing in diabetic neuropathic foot ulcers.

Sequence similarities: Belongs to the PDGF/VEGF growth factor family.