Testing Data
Rabbit PDGF Polyclonal Antibody | anti-PDGF antibody
Rabbit anti PDGF Polyclonal antibody
Reactivity
Human, Rat, Mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
The Rabbit IgG is purified by Epitope Affinity Purification.
Synonyms
PDGF; Polyclonal Antibody; Rabbit anti PDGF Polyclonal antibody; Rabbit anti-PDGF; Platelet-Derived Growth Factor (PDGF); anti-PDGF antibody
Host
Rabbit
Reactivity
Human, Rat, Mouse
Clonality
Polyclonal
Specificity
This antibody recognizes ~23 kDa of human PDGF protein. The other species are not tested.
Purity/Purification
The Rabbit IgG is purified by Epitope Affinity Purification.
Form/Format
This affinity purified antibody is supplied in sterile Phosphate buffered saline (pH7.2) containing antibody stabilizer
Concentration
Size: 100 ug/200 ul (varies by lot)
Applicable Applications for anti-PDGF antibody
Western Blot, Immunohistochemistry, Immunofluorescence
Application Notes
Western Blot: 0.1-1 ug/ml
ELISA: 0.01-0.1 ug/ml
Immunoprecipitation: 2-5 ug/ml
Immunohistochemistry: 2-5 ug/ml
ELISA: 0.01-0.1 ug/ml
Immunoprecipitation: 2-5 ug/ml
Immunohistochemistry: 2-5 ug/ml
Antigen Preparation
A fusion recombinant protein derived from E Coli, containing 196aa- full length of human PDGF protein
Positive Control
Full length PDGF recombinant protein
Preparation and Storage
The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C. The antibodies can be stored at 2 degree C-8 degree C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Testing Data
Testing Data
Related Product Information for anti-PDGF antibody
The platelet-derived growth factor (PDGF) family of disulfide-linked dimeric proteins consists of homo/hetero dimeric proteins, PDGF-AA, -BB, -CC, -DD and -AB. The PDGF-AA, -BB and AB bind to alpha type receptor, while beta type receptors bind to PDGF-BB and PDGF-AB. Upon activation by PDGF, the receptors get dimerised and auphosphorylated in several sites on its receptors (Receptor tyrosine kinase) in cytosolic domain to trigger cell signaling through PI3K pathway. All PDGF isoforms are synthesized as inactive precursors and are processed to active forms.
References
Chen WY, et al (2004) Identification and validation of PDGF transcriptional targets by microarray-coupled genetrap mutagenesis. Nat Genet. 36 (3):304-12.
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Product Notes
The PDGF (Catalog #AAA462205) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti PDGF Polyclonal antibody reacts with Human, Rat, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PDGF can be used in a range of immunoassay formats including, but not limited to, Western Blot, Immunohistochemistry, Immunofluorescence. Western Blot: 0.1-1 ug/ml ELISA: 0.01-0.1 ug/ml Immunoprecipitation: 2-5 ug/ml Immunohistochemistry: 2-5 ug/ml. Researchers should empirically determine the suitability of the PDGF for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PDGF, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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