PDGF Receptor beta Antibody Kit | PDGFRB antibody kit

PDGF Receptor beta Antibody Sampler Kit

Cat. Num. AAA2550135

• Gene Names: PDGFRB; IMF1; KOGS; IBGC4; JTK12; PDGFR; PENTT; CD140B; PDGFR1; PDGFR-1
• Applications: Western Blot
• Synonyms: PDGF Receptor beta; PDGF Receptor beta Antibody Sampler Kit; PDGFRB antibody kit

• Sequence Length: 1106

• Applicable Applications for PDGFRB antibody kit: Western Blot (WB)

Western Blot (WB)

(Western Blot analysis of Hela, KB cells using PDGFR-beta Polyclonal Antibody at dilution of 1:1000.)

Related Product Information for PDGFRB antibody kit

Background: Platelet derived growth factor (PDGF) family proteins exist as several disulphide-bonded, dimeric isoforms (PDGF AA, PDGF AB, PDGF BB, PDGF CC, and PDGF DD) that bind in a specific pattern to two closely related receptor tyrosine kinases, PDGF receptor alpha (PDGFRalpha) and PDGF receptor beta (PDGFRbeta). PDGFRalpha and PDGFRbeta share 75% to 85% sequence homology between their two intracellular kinase domains, while the kinase insert and carboxy-terminal tail regions display a lower level (27% to 28%) of homology. PDGFRalpha homodimers bind all PDGF isoforms except those containing PDGF D. PDGFRbeta homodimers bind PDGF BB and DD isoforms, as well as the PDGF AB heterodimer. The heteromeric PDGF receptor alpha/beta binds PDGF B, C, and D homodimers, as well as the PDGF AB heterodimer. PDGFRalpha and PDGFRbeta can each form heterodimers with EGFR, which is also activated by PDGF. Various cells differ in the total number of receptors present and in the receptor subunit composition, which may account for responsive differences among cell types to PDGF binding. Ligand binding induces receptor dimerization and autophosphorylation, followed by binding and activation of cytoplasmic SH2 domain-containing signal transduction molecules, such as GRB2, Src, GAP, PI3 kinase, PLCgamma, and NCK. A number of different signaling pathways are initiated by activated PDGF receptors and lead to control of cell growth, actin reorganization, migration, and differentiation. Tyr751 in the kinase-insert region of PDGFRbeta is the docking site for PI3 kinase. Phosphorylated pentapeptides derived from Tyr751 of PDGFRbeta (pTyr751-Val-Pro-Met-Leu) inhibit the association of the carboxy-terminal SH2 domain of the p85 subunit of PI3 kinase with PDGFRbeta. Tyr740 is also required for PDGFRbeta-mediated PI3 kinase activation.

NCBI and Uniprot Product Information

• NCBI GI #: 4505683
• NCBI GeneID: 5159
• NCBI Accession #: NP_002600.1
• NCBI GenBank Nucleotide #: NM_002609.3
• UniProt Accession #: P09619
• NCBI Official Full Name: platelet-derived growth factor receptor beta isoform 1
• NCBI Official Synonym Full Names: platelet derived growth factor receptor beta
• NCBI Official Symbol: PDGFRB
• NCBI Official Synonym Symbols: IMF1; KOGS; IBGC4; JTK12; PDGFR; PENTT; CD140B; PDGFR1; PDGFR-1
• NCBI Protein Information: platelet-derived growth factor receptor beta
• Protein Family: Platelet-derived growth factor receptor

NCBI Description

The protein encoded by this gene is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer (PDGFB or PDGFD) or a heterodimer (PDGFA and PDGFB). This gene is essential for normal development of the cardiovascular system and aids in rearrangement of the actin cytoskeleton. This gene is flanked on chromosome 5 by the genes for granulocyte-macrophage colony-stimulating factor and macrophage-colony stimulating factor receptor; all three genes may be implicated in the 5-q syndrome. A translocation between chromosomes 5 and 12, that fuses this gene to that of the ETV6 gene, results in chronic myeloproliferative disorder with eosinophilia. [provided by RefSeq, Aug 2017]