Human Anoctamin 6 (ANO6) ELISA Kit | ANO6 elisa kit

Human Anoctamin 6 (ANO6) ELISA Kit

Cat. Num. AAA2705913

• Gene Names: ANO6; SCTS; BDPLT7; TMEM16F
• Reactivity: Human
• Synonyms: Anoctamin 6 (ANO6); Human Anoctamin 6 (ANO6) ELISA Kit; TMEM16F; SCAN channel; Transmembrane Protein 16F; Small-conductance calcium-activated nonselective cation channel; ANO6 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Anoctamin 6 (ANO6).; No significant cross-reactivity or interference between Anoctamin 6 (ANO6) and analogues was observed.
• Sequence Length: 929

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.127ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Anoctamin 6 (ANO6) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Anoctamin 6 (ANO6) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ANO6 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Anoctamin 6 (ANO6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Anoctamin 6 (ANO6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Anoctamin 6 (ANO6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Anoctamin 6 (ANO6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 68534512
• NCBI GeneID: 196527
• UniProt Accession #: Q4KMQ2; Q8N3Q2; A6NNM6; B9EGG0; E7ENK4; E9PB30; E9PCT2
• Molecular Weight: 108,102 Da
• NCBI Official Full Name: Anoctamin 6
• NCBI Official Synonym Full Names: anoctamin 6
• NCBI Official Symbol: ANO6
• NCBI Official Synonym Symbols: SCTS; BDPLT7; TMEM16F
• NCBI Protein Information: anoctamin-6
• UniProt Protein Name: Anoctamin-6
• Protein Family: Anoctamin
• UniProt Gene Name: ANO6
• UniProt Synonym Gene Names: TMEM16F; SCAN channel

NCBI Description

This gene encodes a multi-pass transmembrane protein that belongs to the anoctamin family. This protein is an essential component for the calcium-dependent exposure of phosphatidylserine on the cell surface. The scrambling of phospholipid occurs in various biological systems, such as when blood platelets are activated, they expose phosphatidylserine to trigger the clotting system. Mutations in this gene are associated with Scott syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2011]

Uniprot Description

Small-conductance calcium-activated nonselective cation (SCAN) channel which acts as a regulator of phospholipid scrambling in platelets and osteoblasts. Phospholipid scrambling results in surface exposure of phosphatidylserine which in platelets is essential to trigger the clotting system whereas in osteoblasts is essential for the deposition of hydroxyapatite during bone mineralization. Has calcium-dependent phospholipid scramblase activity; scrambles phosphatidylserine, phosphatidylcholine and galactosylceramide (). Can generate outwardly rectifying chloride channel currents in airway epithelial cells and Jurkat T lymphocytes.