Rabbit anti-Human PDE9A Polyclonal Antibody | anti-PDE9A antibody

Anti-PDE9A Antibody, Rabbit Polyclonal

Cat. Num. AAA8105768

• Gene Names: PDE9A; FLJ90181; HSPDE9A2; PDE9A
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Protein A & Antigen Affinity
• Synonyms: PDE9A; Polyclonal Antibody; Anti-PDE9A Antibody; Rabbit Polyclonal; PDE9A Antibody; Rabbit PAb; Antigen Affinity Purified; phosphodiesterase 9A; Anti-HSPDE9A2 Antibody; anti-PDE9A antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human PDE9A
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-PDE9A antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:500-1:1000; ELISA: 1:25,000-1:50,000
• Immunogen: Recombinant Human PDE9A Protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Human PDE9A (rh PDE9A). PDE9A specific IgG was purified by Human PDE9A affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-PDE9A rabbit polyclonal antibody at 1:500 dilution.Lane A: Jurkat Whole Cell LysateLane B: 293T Whole Cell LysateLane C: Mouse brain Tissue lysate Lysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:68 kDaObserved band size:68 kDa(We are unsure as to the identity of these extra bands.))

Related Product Information for anti-PDE9A antibody

High affinity cGMP-specific 3', 5'-cyclic phosphodiesterase 9A, also known as PDE9A, is a member of the cyclic nucleotide phosphodiesterase family and PDE9 subfamily. PDE9A is expressed in all tissues examined (testis, brain, small intestine, skeletal muscle, heart, lung, thymus, spleen, placenta, kidney, liver, pancreas, ovary and prostate) except blood. Highest levels of PDE9A is in brain, heart, kidney, spleen, prostate and colon. Isoform PDE9A2 is found in prostate. PDE9A mRNA is widely distributed throughout the rat and mouse brain, with the highest expression observed in cerebellar Purkinje cells. PDE9A is the only cGMP-specific PDE with significant expression in the forebrain, and as such is likely to play an important role in NO-cGMP signaling. PDE9A is highly conserved between species and is widely distributed throughout the rodent brain. PDE9A is probably involved in maintenance of low cGMP levels in cells and might play an important role in a variety of brain functions involving cGMP-mediated signal transduction. PDE9A hydrolyzes the second messenger cGMP, which is a key regulator of many important physiological processes. PDE9A represents a novel drug target worthy of further study.

References

Andreeva, SG. et al., 2001, J Neurosci. 21 (22):9068-76. van Staveren, WC. et al., 2002, J Neurocytol. 31 (8-9):729-41. Almeida, CB. et al., 2008, Br J Haematol. 142 (5):836-44. Kruse, L.S. et al., 2009, Brain Res. 1281 :25-34.

NCBI and Uniprot Product Information

• NCBI GI #: 48762718
• NCBI GeneID: 5152
• UniProt Accession #: O76083; O75490; O75491; O95225; Q53Y40; Q5QD39; Q86SF7; Q86SI6; Q86SJ3; Q86WN3; B2RBI5; B4DFI5
• NCBI Official Full Name: high affinity cGMP-specific 3',5'-cyclic phosphodiesterase 9A isoform b [Homo sapiens]
• NCBI Official Synonym Full Names: phosphodiesterase 9A
• NCBI Official Symbol: PDE9A
• NCBI Official Synonym Symbols: FLJ90181; HSPDE9A2; PDE9A
• UniProt Protein Name: High affinity cGMP-specific 3',5'-cyclic phosphodiesterase 9A
• Protein Family: High affinity cGMP-specific 3',5'-cyclic phosphodiesterase
• UniProt Gene Name: PDE9A
• UniProt Entry Name: PDE9A_HUMAN