Testing Data
(The cell lysates of Jurkat (30 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with MAFK antibody (1:1000).)
Mouse MAFK Monoclonal Antibody | anti-MAFK antibody
MAFK antibody
Applications
ELISA, Western Blot
Purity
Purified by protein-A affinity chromatography
Synonyms
MAFK; Monoclonal Antibody; MAFK antibody; Mouse monoclonal MAFK antibody; Monoclonal MAFK antibody; Anti-MAFK antibody; Transcription factor MafK antibody; NFE2U antibody; P18 antibody; anti-MAFK antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2a kappa
Clone Number
AT2F7
Purity/Purification
Purified by protein-A affinity chromatography
Form/Format
Liquid form in Phosphate-Buffered Saline (pH 7.4) containing 10% Glycerol and 0.02% Sodium Azide
Concentration
1 mg/ml (varies by lot)
Sequence Length
162
Applicable Applications for anti-MAFK antibody
ELISA (EIA), Western Blot (WB)
Application Notes
ELISA: 1:1000
Immunogen
MAFK antibody was raised in Mouse using recombinant human MAFK (1-156aa) purified from E Coli as the immunogen
Preparation and Storage
Can be stored at 4 degree C short term. For long term storage, aliquot and store at-20 degree C.
Avoid repeated freezing and thawing cycles.
Avoid repeated freezing and thawing cycles.
Testing Data
(The cell lysates of Jurkat (30 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with MAFK antibody (1:1000).)
Testing Data
(The cell lysates of Jurkat (30 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with MAFK antibody (1:1000).)
Related Product Information for anti-MAFK antibody
The developmentally regulated expression of the globin genes depends on upstream regulatory elements termed locus control regions (LCRs). LCRs are associated with powerful enhancer activity that is mediated by the transcription factor NFE2 (nuclear factor erythroid-2). NFE2 DNA-binding activity consists of a heterodimer containing an 18-kD Maf protein (MafF, MafG or MafK) and p45. Since Maf homodimers lack a putative transactivation domain, the small Mafs behave as transcriptional repressors when they dimerize among themselves. However, they seem to serve as transcriptional activators by dimerizing with other (usually larger) basic-zipper proteins and recruiting them to specific DNA-binding sites. Small Maf proteins heterodimerize with Fos and may act as competitive repressors of the NF-E2 transcription factor. The MafK of Maf proteins is primarily expressed during development in mesenchymal and hematopoietic cells and neurons.
Product Categories/Family for anti-MAFK antibody
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
18,485 Da
NCBI Official Full Name
MAFK, partial
UniProt Protein Name
MAFK
Protein Family
UniProt Gene Name
MAFK
Similar Products
Product Notes
The MAFK mafk (Catalog #AAA5310101) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's MAFK can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). ELISA: 1:1000. Researchers should empirically determine the suitability of the MAFK mafk for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MAFK, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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