Rabbit anti-Human PDE7B Polyclonal Antibody | anti-PDE7B antibody

PDE7B, CT (cAMP-specific 3',5'-cyclic Phosphodiesterase 7B)

Cat. Num. AAA649267

• Gene Names: PDE7B; bA472E5.1
• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: PDE7B; Polyclonal Antibody; CT (cAMP-specific 3'; 5'-cyclic Phosphodiesterase 7B); Anti -PDE7B; anti-PDE7B antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Recognizes human PDE7B. Species sequence homology: mouse (58%) and rat (63%).
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.1% sodium azide.

• Applicable Applications for anti-PDE7B antibody: Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Immunohistochemistry.; Dilution: Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml
• Immunogen: Synthetic peptide corresponding to 19aa from C-terminal cytoplasmic domain of human GPRC5C.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Human Pancreas (formalin-fixed, paraffin-embedded) stained with PDE7B antibody at 5ug/ml followed by biotinylated goat rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.)

Related Product Information for anti-PDE7B antibody

Phosphodiesterase 7B, a cAMP-specific phosphodiesterase, has been shown to affect cAMP-mediated neural activity and cAMP metabolism in the brain. PDE7B activity is inhibited by dipyridamole, IBMX, and SCH51866. At least three splice variants have been isolated for PDE7B from rats and they show tissue specific expression patterns.

Product Categories/Family for anti-PDE7B antibody

Antibodies; Abs to Enzymes; Phosphodiesterase

NCBI and Uniprot Product Information

• NCBI GI #: 47123461
• NCBI GeneID: 27115
• UniProt Accession #: Q9NP56; Q5W154
• Molecular Weight: 51,835 Da
• NCBI Official Full Name: PDE7B protein
• NCBI Official Synonym Full Names: phosphodiesterase 7B
• NCBI Official Symbol: PDE7B
• NCBI Official Synonym Symbols: bA472E5.1
• NCBI Protein Information: cAMP-specific 3',5'-cyclic phosphodiesterase 7B; rolipram-insensitive phosphodiesterase type 7; high-affinity cAMP-specific 3',5'-cyclic phosphodiesterase
• UniProt Protein Name: cAMP-specific 3',5'-cyclic phosphodiesterase 7B
• Protein Family: cAMP-specific 3',5'-cyclic phosphodiesterase
• UniProt Gene Name: PDE7B
• UniProt Entry Name: PDE7B_HUMAN

NCBI Description

The 3',5'-cyclic nucleotides cAMP and cGMP function as second messengers in a wide variety of signal transduction pathways. 3',5'-cyclic nucleotide phosphodiesterases (PDEs) catalyze the hydrolysis of cAMP and cGMP to the corresponding 5'-monophosphates and provide a mechanism to downregulate cAMP and cGMP signaling. This gene encodes a cAMP-specific phosphodiesterase, a member of the cyclic nucleotide phosphodiesterase family.[provided by RefSeq, Apr 2009]

Uniprot Description

Function: Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. May be involved in the control of cAMP-mediated neural activity and cAMP metabolism in the brain.

Catalytic activity: Adenosine 3',5'-cyclic phosphate + H2O = adenosine 5'-phosphate.

Cofactor: Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions

By similarity.

Enzyme regulation: Inhibited by dipyridamole, IBMX and SCH 51866. Insensitive to zaprinast, rolipram, and milrinone.

Pathway: Purine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1.

Tissue specificity: Highly expressed in brain. Also expressed in heart, liver, skeletal muscle and pancreas.

Domain: Composed of a C-terminal catalytic domain containing two putative divalent metal sites and an N-terminal regulatory domain.

Sequence similarities: Belongs to the cyclic nucleotide phosphodiesterase family. PDE7 subfamily.