Rabbit anti-Mouse PDE6H Polyclonal Antibody | anti-PDE6H antibody

PDE6H, ID (PDE6H, Retinal cone rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit gamma) (AP)

Cat. Num. AAA6332188

• Gene Names: PDE6H; RCD3; ACHM6
• Reactivity: Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PDE6H; Polyclonal Antibody; ID (PDE6H; Retinal cone rhodopsin-sensitive cGMP 3'; 5'-cyclic phosphodiesterase subunit gamma) (AP); 5'-cyclic phosphodiesterase subunit gamma; anti-PDE6H antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-PDE6H antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: PDE6H antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 4-33 amino acids from the Central region of human PDE6H.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(PDE6H Antibody (Center) western blot analysis in mouse testis tissue lysates (35ug/lane).This demonstrates the PDE6H antibody detected the PDE6H protein (arrow).)

Related Product Information for anti-PDE6H antibody

This gene encodes the inhibitory (or gamma) subunit of the cone-specific cGMP phosphodiesterase, which is a tetramer composed of two catalytic chains (alpha and beta), and two inhibitory chains (gamma). It is specifically expressed in the retina, and is involved in the transmission and amplification of the visual signal. Mutations in this gene are associated with retinal cone dystrophy type 3A (RCD3A).

Product Categories/Family for anti-PDE6H antibody

Antibodies; Enzymes; Phosphodiesterase

References

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Lamesch, P., et al. Genomics 89(3):307-315(2007). Piri, N., et al. Ophthalmology 112(1):159-166(2005). Kajimura, N., et al. J. Struct. Biol. 139(1):27-38(2002). Shimizu-Matsumoto, A., et al. Genomics 32(1):121-124(1996).

NCBI and Uniprot Product Information

• NCBI GI #: 5453868
• NCBI GeneID: 5149
• NCBI Accession #: NP_006196.1
• NCBI GenBank Nucleotide #: NM_006205.2
• UniProt Accession #: Q13956; Q52LY7
• Molecular Weight: 9,074 Da
• NCBI Official Full Name: retinal cone rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit gamma
• NCBI Official Synonym Full Names: phosphodiesterase 6H
• NCBI Official Symbol: PDE6H
• NCBI Official Synonym Symbols: RCD3; ACHM6
• NCBI Protein Information: retinal cone rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit gamma
• UniProt Protein Name: Retinal cone rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit gamma
• Protein Family: Retinal cone rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase
• UniProt Gene Name: PDE6H
• UniProt Synonym Gene Names: GMP-PDE gamma
• UniProt Entry Name: CNCG_HUMAN

Uniprot Description

PDE6H: Participates in processes of transmission and amplification of the visual signal. cGMP-PDEs are the effector molecules in G-protein-mediated phototransduction in vertebrate rods and cones. Defects in PDE6H are the cause of cone dystrophy retinal type 3A (RCD3A); also known as cone dystrophy with night blindness and supernormal rod responses. RCD3A is a rare form of cone dystrophy associated with supernormal rod responses. The disorder is characterized by reduced visual acuity, photoaversion, night blindness, and abnormal color vision. At an early age, the retina shows subtle depigmentation at the macula and, later, more obvious areas of atrophy. Belongs to the rod/cone cGMP-PDE gamma subunit family.

Protein type: EC 3.1.4.35; Inhibitor; Nucleotide Metabolism - purine; Phosphodiesterase

Chromosomal Location of Human Ortholog: 12p13

Molecular Function: enzyme inhibitor activity; protein binding

Disease: Retinal Cone Dystrophy 3a