Human Nucleolysin TIA-1 isoform p40, TIA1 ELISA Kit | TIA1 elisa kit

Human Nucleolysin TIA-1 isoform p40, TIA1 ELISA Kit

Cat. Num. AAA2602700

• Gene Names: TIA1; WDM; TIA-1
• Reactivity: Human
• Synonyms: Nucleolysin TIA-1 isoform p40; TIA1; Human Nucleolysin TIA-1 isoform p40; TIA1 ELISA Kit; TIA1 elisa kit

• Reactivity: Human
• Specificity: No cross-reaction with other factors.

• Samples: Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant TIA1 concentration
• Assay Type: Sandwich
• Detection Range: 20 ng/ml-0.312 ng/ml
• Sensitivity: 0.06 ng/ml.
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TIA1 elisa kit

Priciple of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human TIA1 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 188219593
• NCBI GeneID: 7072
• NCBI Accession #: NP_071320.2
• NCBI GenBank Nucleotide #: NM_022037.2
• UniProt Accession #: P31483; Q53SS9
• Molecular Weight: 42,963 Da
• NCBI Official Full Name: nucleolysin TIA-1 isoform p40 isoform 1
• NCBI Official Synonym Full Names: TIA1 cytotoxic granule-associated RNA binding protein
• NCBI Official Symbol: TIA1
• NCBI Official Synonym Symbols: WDM; TIA-1
• NCBI Protein Information: nucleolysin TIA-1 isoform p40; p40-TIA-1 (containing p15-TIA-1); T-cell-restricted intracellular antigen-1
• UniProt Protein Name: Nucleolysin TIA-1 isoform p40
• Protein Family: Nucleolysin
• UniProt Gene Name: TIA1
• UniProt Synonym Gene Names: TIA-1
• UniProt Entry Name: TIA1_HUMAN

NCBI Description

The product encoded by this gene is a member of a RNA-binding protein family and possesses nucleolytic activity against cytotoxic lymphocyte (CTL) target cells. It has been suggested that this protein may be involved in the induction of apoptosis as it preferentially recognizes poly(A) homopolymers and induces DNA fragmentation in CTL targets. The major granule-associated species is a 15-kDa protein that is thought to be derived from the carboxyl terminus of the 40-kDa product by proteolytic processing. Alternative splicing resulting in different isoforms of this gene product has been described in the literature. [provided by RefSeq, Jul 2008]

Uniprot Description

TIA1: Involved in alternative pre-RNA splicing and regulation of mRNA translation by binding to AU-rich elements (AREs) located in mRNA 3' untranslated regions (3' UTRs). Possesses nucleolytic activity against cytotoxic lymphocyte target cells. May be involved in apoptosis. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Translation; RNA-binding

Chromosomal Location of Human Ortholog: 2p13

Cellular Component: nucleoplasm; stress granule; cytoplasm

Molecular Function: protein binding; nucleotide binding; AU-rich element binding; poly(A) binding

Biological Process: regulation of nuclear mRNA splicing, via spliceosome; apoptosis; negative regulation of translation; negative regulation of cytokine biosynthetic process

Disease: Welander Distal Myopathy