Western Blot (WB)
(Anti-PDE4D rabbit polyclonal antibody at 1:500 dilution Lane A: K562 Whole Cell Lysate Lane B: HeLa Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:91 kDa Observed band size:110 kDa)
Rabbit anti-Human PDE4D Polyclonal Antibody | anti-PDE4D antibody
Anti-PDE4D Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Western Blot, Immunoprecipitation
Purity
Protein A & Antigen Affinity
Synonyms
PDE4D; Polyclonal Antibody; Anti-PDE4D Antibody; Rabbit Polyclonal; PDE4D Antibody; Rabbit PAb; Antigen Affinity Purified; phosphodiesterase 4D; cAMP-specific; Anti-ACRDYS2 Antibody; Anti-DPDE3 Antibody; Anti-HSPDE4D Antibody; Anti-PDE43 Antibody; Anti-PDE4DN2 Antibody; Anti-STRK1 Antibody; anti-PDE4D antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human PDE4D
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-PDE4D antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes
WB: 1:500-1:2000
IP: 1-5uL/mg of lysate
IP: 1-5uL/mg of lysate
Immunogen
E Coli-derived Human PDE4D fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human PDE4D fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Anti-PDE4D rabbit polyclonal antibody at 1:500 dilution Lane A: K562 Whole Cell Lysate Lane B: HeLa Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:91 kDa Observed band size:110 kDa)
Western Blot (WB)
(Anti-PDE4D rabbit polyclonal antibody at 1:500 dilution Lane A: K562 Whole Cell Lysate Lane B: HeLa Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:91 kDa Observed band size:110 kDa)
Immunoprecipitation (IP)
(PDE4D was immunoprecipitated using: Lane A:0.5 mg HeLa Whole Cell Lysate 4 uL anti-PDE4D rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-PDE4D rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 91 kDa Observed band size :100 kDa)
Immunoprecipitation (IP)
(PDE4D was immunoprecipitated using: Lane A:0.5 mg HeLa Whole Cell Lysate 4 uL anti-PDE4D rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-PDE4D rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 91 kDa Observed band size :100 kDa)
Related Product Information for anti-PDE4D antibody
PDE4D has been reported as an oncogene in various types of human cancers. Mutations in the gene encoding phosphodiesterase 4D (PDE4D) enzyme are associated with ischemic stroke. PDE4D is part of a complex protein family modulating intracellular signaling by cyclic nucleotides. The PDE4 family includes subtypes A-D, all of which show unique intracellular, cellular, and tissue distribution. PDE4D is the major subtype expressed in human atrial myocytes and involved in the pathophysiology of arrhythmias, such as atrial fibrillation. The PDE4D enzyme hydrolyses cyclic adenosine monophosphate (cAMP). Mouse Pde4d is located on chromosome 13 and serves many functions in important physiological processes involving cyclic adenosine monophosphate. Pde4d might serve specific biological functions in regulating the development process of the mouse embryo, and that CpG methylation of the Pde4d promoter may play an important role in regulating Pde4d at a transcriptional level.
References
Liu F, et al. (2019) High expression of pde4d correlates with poor prognosis and clinical progression in pancreaticductal adenocarcinoma. J Cancer 10 (25): 6252-6260.Jorgensen C, et al. (2015) Phosphodiesterase4d (pde4d)--a risk factor for atrial fibrillation and stroke? J Neurol Sci 359 (1-2): 266-274.Huang Z, et al. (2013) Dynamic expression pattern of pde4d and its relationship with cpg methylation in the promoter during mouse embryo development. Biochem Biophys Res Commun 441 (4): 982-987.
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Product Notes
The PDE4D (Catalog #AAA8109256) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PDE4D Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PDE4D can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP). WB: 1:500-1:2000 IP: 1-5uL/mg of lysate. Researchers should empirically determine the suitability of the PDE4D for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PDE4D, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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