Rabbit anti-Human PDCD6IP Polyclonal Antibody | anti-PDCD6IP antibody

PDCD6IP, ID (PDCD6IP, AIP1, ALIX, KIAA1375, Programmed cell death 6-interacting protein, ALG-2-interacting protein 1, Hp95) (MaxLight 750)

Cat. Num. AAA6332043

• Gene Names: PDCD6IP; AIP1; ALIX; HP95; DRIP4
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: PDCD6IP; Polyclonal Antibody; ID (PDCD6IP; AIP1; ALIX; KIAA1375; Programmed cell death 6-interacting protein; ALG-2-interacting protein 1; Hp95) (MaxLight 750); Hp95; anti-PDCD6IP antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PDCD6IP.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight750.

• Applicable Applications for anti-PDCD6IP antibody: Western Blot (WB), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 541-570 in the central region of human PDCD6IP.
• Conjugate: MaxLight750
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight750 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in ZR-75-1 cell line lysates(35ug/lane) usingMBS649545. This demonstrates that MBS649545 detected the PDCD6IP protein (arrow).)

Related Product Information for anti-PDCD6IP antibody

This gene encodes a protein thought to participate in programmed cell death. Studies using mouse cells have shown that overexpression of this protein can block apoptosis. In addition, the product of this gene binds to the product of the PDCD6 gene, a protein required for apoptosis, in a calcium-dependent manner. This gene product also binds to endophilins, proteins that regulate membrane shape during endocytosis. Overexpression of this gene product and endophilins results in cytoplasmic vacuolization, which may be partly responsible for the protection against cell death. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].

Product Categories/Family for anti-PDCD6IP antibody

Antibodies; Apoptosis

References

Shi, X., et al. Biochem. J. 431(1):93-102(2010). Irie, T., et al. Virology 405(2):334-341(2010). Sette, P., et al. J. Virol. 84(16):8181-8192(2010). Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Inuzuka, T., et al. BMC Struct. Biol. 10, 25 (2010).

NCBI and Uniprot Product Information

• NCBI GI #: 22027538
• NCBI GeneID: 10015
• NCBI Accession #: NP_037506.2
• NCBI GenBank Nucleotide #: NM_013374.5
• UniProt Accession #: Q8WUM4; Q6NUS1; Q9BX86; Q9NUN0; Q9P2H2; Q9UKL5; C5MQH7; E9PFU1
• Molecular Weight: 30,453 Da
• NCBI Official Full Name: programmed cell death 6-interacting protein isoform 1
• NCBI Official Synonym Full Names: programmed cell death 6 interacting protein
• NCBI Official Symbol: PDCD6IP
• NCBI Official Synonym Symbols: AIP1; ALIX; HP95; DRIP4
• NCBI Protein Information: programmed cell death 6-interacting protein
• UniProt Protein Name: Programmed cell death 6-interacting protein
• Protein Family: Programmed cell death 6-interacting protein
• UniProt Gene Name: PDCD6IP
• UniProt Synonym Gene Names: AIP1; ALIX; KIAA1375; PDCD6-interacting protein
• UniProt Entry Name: PDC6I_HUMAN

NCBI Description

This gene encodes a protein that functions within the ESCRT pathway in the abscission stage of cytokinesis, in intralumenal endosomal vesicle formation, and in enveloped virus budding. Studies using mouse cells have shown that overexpression of this protein can block apoptosis. In addition, the product of this gene binds to the product of the PDCD6 gene, a protein required for apoptosis, in a calcium-dependent manner. This gene product also binds to endophilins, proteins that regulate membrane shape during endocytosis. Overexpression of this gene product and endophilins results in cytoplasmic vacuolization, which may be partly responsible for the protection against cell death. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. Related pseudogenes have been identified on chromosome 15. [provided by RefSeq, Jan 2012]

Uniprot Description

Alix: an adaptor protein that may regulate the function of receptor and cytoskeleton-associated tyrosine kinases. A class E vacuolar protein sorting (VPS) factor involved in concentration and sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles. Fusion between endosomes and the vacuole will then target the cargo proteins to the vacuolar lumen. In case of infection, the HIV-1 virus directly takes advantage of its function for viral exocytosis and budding, via its interaction with HIV-1 p6 protein. May play a role in the regulation of both apoptosis and cell proliferation.

Protein type: Apoptosis; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 3p22.3

Cellular Component: focal adhesion; membrane; cytoplasm; melanosome; microtubule organizing center; immunological synapse; cytosol; vesicle

Molecular Function: protein binding; protein homodimerization activity; proteinase activated receptor binding; SH3 domain binding; calcium-dependent protein binding

Biological Process: protein transport; cell separation during cytokinesis; viral reproduction; apoptosis; viral infectious cycle; mitotic metaphase plate congression; nuclear organization and biogenesis