Western Blot (WB)
(PCSK6 Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the PCSK6 antibody detected the PCSK6 protein (arrow).)
Rabbit anti-Human PCSK6 Polyclonal Antibody | anti-PCSK6 antibody
PCSK6, CT (PCSK6, PACE4, Proprotein convertase subtilisin/kexin type 6, Paired basic amino acid cleaving enzyme 4, Subtilisin-like proprotein convertase 4, Subtilisin/kexin-like protease PACE4)
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Western Blot (WB)
(PCSK6 Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the PCSK6 antibody detected the PCSK6 protein (arrow).)
Western Blot (WB)
(PCSK6 Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the PCSK6 antibody detected the PCSK6 protein (arrow).)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene belongs to the subtilisin-like proprotein convertase family. The members of this family are proprotein convertases that process latent precursor proteins into their biologically active products. This encoded protein is a calcium-dependent serine endoprotease that can cleave precursor protein at their paired basic amino acid processing sites. Some of its substrates are - transforming growth factor beta related proteins, proalbumin, and von Willebrand factor. This gene is thought to play a role in tumor progression. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
Uniprot Description
Function: Likely to represent an endoprotease activity within the constitutive secretory pathway, with unique restricted distribution in both neuroendocrine and non-neuroendocrine tissues and capable of cleavage at the RX(K/R)R consensus motif.
Catalytic activity: Release of mature proteins from their proproteins by cleavage of Arg-Xaa-Yaa-Arg-|-Zaa bonds, where Xaa can be any amino acid and Yaa is Arg or Lys.
Cofactor: Calcium
Potential.
Subunit structure: The PACE4A-I precursor protein seems to exist in the reticulum endoplasmic as both a monomer and a dimer-sized complex whereas mature PACE4A-I exists only as a monomer, suggesting that propeptide cleavage affects its tertiary or quaternary structure.
Subcellular location: Isoform PACE4A-I: Secreted. Isoform PACE4A-II: Secreted. Isoform PACE4C: Endoplasmic reticulum. Note: Not secreted, remains probably in zymogen form in endoplasmic reticulum.Isoform PACE4CS: Endoplasmic reticulum. Note: Not secreted, remains probably in zymogen form in endoplasmic reticulum.Isoform PACE4E-I: Endomembrane system; Peripheral membrane protein. Note: Retained intracellularly probably through a hydrophobic cluster in their C-terminus.Isoform PACE4E-II: Endomembrane system; Peripheral membrane protein. Note: Retained intracellularly probably through a hydrophobic cluster in their C-terminus.Isoform PACE4B: Secreted.
Tissue specificity: Each PACE4 isoform exhibits a unique restricted distribution. Isoform PACE4A-I is expressed in heart, brain, placenta, lung, skeletal muscle, kidney, pancreas, but at comparatively higher levels in the liver. Isoform PACE4A-II is at least expressed in placenta. Isoform PACE4B was only found in the embryonic kidney cell line from which it was isolated. Isoform PACE4C and isoform PACE4D are expressed in placenta. Isoform PACE4E-I is expressed in cerebellum, placenta and pituitary. Isoform PACE4E-II is at least present in cerebellum.
Domain: The propeptide domain acts as an intramolecular chaperone assisting the folding of the zymogen within the endoplasmic reticulum. Isoform PACE4D lacks the propeptide domain.
Sequence similarities: Belongs to the peptidase S8 family.Contains 1 homo B/P domain.Contains 1 PLAC domain.
Research Articles on PCSK6
Similar Products
Product Notes
The PCSK6 pcsk6 (Catalog #AAA647538) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PCSK6, CT (PCSK6, PACE4, Proprotein convertase subtilisin/kexin type 6, Paired basic amino acid cleaving enzyme 4, Subtilisin-like proprotein convertase 4, Subtilisin/kexin-like protease PACE4) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PCSK6 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in Western Blot, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500. Researchers should empirically determine the suitability of the PCSK6 pcsk6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PCSK6, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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