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Western Blot (WB) (IREB2 Antibody (Center) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the IREB2 antibody detected the IREB2 protein (arrow).)

Rabbit anti-Human IREB2 Polyclonal Antibody | anti-IREB2 antibody

IREB2, ID (IREB2, Iron-responsive element-binding protein 2, Iron regulatory protein 2) (Biotin)

Gene Names
IREB2; ACO3; IRP2; IRP2AD
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, ELISA, Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
IREB2; Polyclonal Antibody; ID (IREB2; Iron-responsive element-binding protein 2; Iron regulatory protein 2) (Biotin); Iron regulatory protein 2; anti-IREB2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Applicable Applications for anti-IREB2 antibody
Flow Cytometry (FC/FACS), ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
FC/FACS: 1:10-50
ELISA: 1:1,000
IHC: 1:10-50
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
IREB2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 208-238 amino acids from the Central region of human IREB2.
Conjugate
Biotin
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(IREB2 Antibody (Center) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the IREB2 antibody detected the IREB2 protein (arrow).)

Western Blot (WB) (IREB2 Antibody (Center) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the IREB2 antibody detected the IREB2 protein (arrow).)

Immunohistochemistry (IHC)

(IREB2 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of IREB2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (IREB2 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of IREB2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(IREB2 Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Flow Cytometry (FC/FACS) (IREB2 Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-IREB2 antibody
IREB2 is a RNA-binding protein that binds to iron-responsive elements (IRES), which are stem-loop structures found in the 5'-UTR of ferritin, and delta aminolevulinic acid synthase mRNAs, and in the 3'-UTR of transferrin receptor mRNA. Binding to the IRE element in ferritin results in the repression of its mRNA translation. Binding of the protein to the transferrin receptor mRNA inhibits the degradation of this otherwise rapidly degraded mRNA.
Product Categories/Family for anti-IREB2 antibody
References
Ucisik-Akkaya, E., et al. Mol. Hum. Reprod. 16(10):770-777(2010). Hansen, H.M., et al. Hum. Mol. Genet. 19(18):3652-3661(2010). Cho, M.H., et al. Nat. Genet. 42(3):200-202(2010). Salahudeen, A.A., et al. Science 326(5953):722-726(2009). DeMeo, D.L., et al. Am. J. Hum. Genet. 85(4):493-502(2009).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
37,487 Da
NCBI Official Full Name
iron-responsive element-binding protein 2
NCBI Official Synonym Full Names
iron-responsive element binding protein 2
NCBI Official Symbol
IREB2
NCBI Official Synonym Symbols
ACO3; IRP2; IRP2AD
NCBI Protein Information
iron-responsive element-binding protein 2; IRE-BP 2; iron regulatory protein 2
UniProt Protein Name
Iron-responsive element-binding protein 2
UniProt Gene Name
IREB2
UniProt Synonym Gene Names
IRE-BP 2; IRP2
UniProt Entry Name
IREB2_HUMAN

Uniprot Description

IREB2: RNA-binding protein that binds to iron-responsive elements (IRES), which are stem-loop structures found in the 5'- UTR of ferritin, and delta aminolevulinic acid synthase mRNAs, and in the 3'-UTR of transferrin receptor mRNA. Binding to the IRE element in ferritin results in the repression of its mRNA translation. Binding of the protein to the transferrin receptor mRNA inhibits the degradation of this otherwise rapidly degraded mRNA. Belongs to the aconitase/IPM isomerase family.

Protein type: RNA-binding; Translation

Chromosomal Location of Human Ortholog: 15q25.1

Cellular Component: mitochondrion; cytoplasm; cytosol

Molecular Function: protein binding; iron-responsive element binding; translation repressor activity; RNA binding; metal ion binding; 4 iron, 4 sulfur cluster binding

Biological Process: erythrocyte homeostasis; response to retinoic acid; cellular iron ion homeostasis; negative regulation of translation; response to iron(II) ion; osteoclast differentiation; iron ion transport; protoporphyrinogen IX biosynthetic process; intestinal absorption; aging; post-embryonic development

Research Articles on IREB2

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Product Notes

The IREB2 ireb2 (Catalog #AAA6310874) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The IREB2, ID (IREB2, Iron-responsive element-binding protein 2, Iron regulatory protein 2) (Biotin) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's IREB2 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB). FC/FACS: 1:10-50 ELISA: 1:1,000 IHC: 1:10-50 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the IREB2 ireb2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IREB2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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