Loading...

Skip to main content

Call us on + 1 (800) 604-9114 for more information about our products

Looking for specific datasheet Manual/COA/MSDS?
Request a Manual/COA/MSDS

Interested to get a quote about our products?
Request a Quote

Western Blot (WB) (Western Blot analysis of various samples using Proliferating Cell Nuclear Antigen Polyclonal Antibody at dilution of 1:1000.)

Rabbit PCNA Polyclonal Antibody | anti-PCNA antibody

PCNA Polyclonal Antibody

Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Affinity Purification
Synonyms
PCNA; Polyclonal Antibody; PCNA Polyclonal Antibody; ATLD2; cb16; Cyclin; DNA polymerase delta auxiliary protein; etID36690.10; fa28e03; fb36g03; HGCN8729; MGC8367; Mutagen-sensitive 209 protein; OTTHUMP00000030189; OTTHUMP00000030190; Pcna/cyclin; PCNAR; Polymerase delta accessory protein; Proliferating cell nuclear antigen; wu:fa28e03; wu:fb36g03; anti-PCNA antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purification
Form/Format
PBS with 0.02% sodium azide, 100 mug/ml BSA and 50% glycerol.
Concentration
470 mug/mL (varies by lot)
Sequence Length
338
Applicable Applications for anti-PCNA antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:2000
IHC: 1:1000-1:2000
Immunogen
Recombinant protein corresponding to Mouse PCNA
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of various samples using Proliferating Cell Nuclear Antigen Polyclonal Antibody at dilution of 1:1000.)

Western Blot (WB) (Western Blot analysis of various samples using Proliferating Cell Nuclear Antigen Polyclonal Antibody at dilution of 1:1000.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded human lung cancer using Proliferating Cell Nuclear Antigen Polyclonal Antibody at dilution of 1:1000.)

Immunohistochemistry (IHC) (Immunohistochemistry analysis of paraffin-embedded human lung cancer using Proliferating Cell Nuclear Antigen Polyclonal Antibody at dilution of 1:1000.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded Mouse spleen using Proliferating Cell Nuclear Antigen Polyclonal Antibody at dilution of 1:1000.)

Immunohistochemistry (IHC) (Immunohistochemistry analysis of paraffin-embedded Mouse spleen using Proliferating Cell Nuclear Antigen Polyclonal Antibody at dilution of 1:1000.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded Rat lung using Proliferating Cell Nuclear Antigen Polyclonal Antibody at dilution of 1:1000.)

Immunohistochemistry (IHC) (Immunohistochemistry analysis of paraffin-embedded Rat lung using Proliferating Cell Nuclear Antigen Polyclonal Antibody at dilution of 1:1000.)
Related Product Information for anti-PCNA antibody
Proliferating Cell Nuclear Antigen, commonly known as PCNA, is a protein that acts as a processivity factor for DNA polymerase delta in eukaryotic cells. This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. PCNA induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. It has to be loaded onto DNA in order to be able to stimulate APEX2. PCNA protein is highly conserved during evolution; the deduced amino acid sequences of rat and human differ by only 4 of 261 amino acids. PCNA has been used as loading control for proliferating cells.
Product Categories/Family for anti-PCNA antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Calculated: 36kDa
Observed: 36kDa
NCBI Official Full Name
TFIIH/NER complex subunit TFB4
NCBI Official Symbol
TFB4
NCBI Protein Information
TFIIH/NER complex subunit TFB4
UniProt Protein Name
General transcription and DNA repair factor IIH subunit TFB4
Protein Family
UniProt Gene Name
TFB4
UniProt Synonym Gene Names
TFIIH subunit TFB4

Uniprot Description

Component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to TFIIK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module TFIIK controls the initiation of transcription.

Similar Products

Product Notes

The PCNA tfb4 (Catalog #AAA2556303) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PCNA Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PCNA can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:2000 IHC: 1:1000-1:2000. Researchers should empirically determine the suitability of the PCNA tfb4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PCNA, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

Item has been added to Shopping Cart

If you are ready to order, navigate to Shopping Cart and get ready to checkout.

Looking for a specific manual?
Request a Manual

Request more Information

Please complete the form below and a representative will contact you as soon as possible.

Request a Manual

Please complete the form below and a representative will contact you as soon as possible.

Request a Quote

Please complete the form below and a representative will contact you as soon as possible.