Rabbit anti-Human PCK2 Polyclonal Antibody | anti-PCK2 antibody

PCK2 Antibody

Cat. Num. AAA7003869

• Gene Names: PCK2; PEPCK; PEPCK2; PEPCK-M
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: >95%,Protein G purified
• Synonyms: PCK2; Polyclonal Antibody; PCK2 Antibody; Phosphoenolpyruvate carboxykinase [GTP]; mitochondrial; PEPCK-M; PEPCK2; anti-PCK2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%,Protein G purified
• Form/Format: Liquid
• Sequence Length: 441

• Applicable Applications for anti-PCK2 antibody: ELISA (EIA), Western Blot (WB)
• Immunogen: Recombinant human Phosphoenolpyruvate carboxykinase [GTP], mitochondrial protein
• Conjugation: Non-conjugated
• Storage Buffer: Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-28477 / sc-130225 / sc-32879
• Preparation and Storage: Shipped at 4 degree C. Upon delivery, aliquot and store at -20 degree C or -80 degree C.

Western Blot (WB)

(Western BlotPositive WB detected in:Hela whole cell lysate,Mouse heart tissueAll lanes: PCK2 antibody at 3.4ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 71,48 kDaObserved band size: 71 kDa)

Related Product Information for anti-PCK2 antibody

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.

NCBI and Uniprot Product Information

• NCBI GI #: 614458223
• NCBI GeneID: 5106
• NCBI Accession #: NP_001018083.2
• NCBI GenBank Nucleotide #: NM_001018073.2
• UniProt Accession #: Q16822; O43253; Q86U01; Q9BV62
• Molecular Weight: 47,564 Da
• NCBI Official Full Name: phosphoenolpyruvate carboxykinase
• NCBI Official Synonym Full Names: phosphoenolpyruvate carboxykinase 2, mitochondrial
• NCBI Official Symbol: PCK2
• NCBI Official Synonym Symbols: PEPCK; PEPCK2; PEPCK-M
• NCBI Protein Information: phosphoenolpyruvate carboxykinase [GTP], mitochondrial
• UniProt Protein Name: Phosphoenolpyruvate carboxykinase [GTP], mitochondrial
• Protein Family: Protein kinase
• UniProt Gene Name: PCK2
• UniProt Synonym Gene Names: PEPCK2; PEPCK-M
• UniProt Entry Name: PCKGM_HUMAN

NCBI Description

This gene encodes a mitochondrial enzyme that catalyzes the conversion of oxaloacetate to phosphoenolpyruvate in the presence of guanosine triphosphate (GTP). A cytosolic form of this protein is encoded by a different gene and is the key enzyme of gluconeogenesis in the liver. Alternatively spliced transcript variants have been described. [provided by RefSeq, Apr 2014]

Uniprot Description

PCK2: Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle. Monomer. Belongs to the phosphoenolpyruvate carboxykinase [GTP] family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Carbohydrate Metabolism - citrate (TCA) cycle; EC 4.1.1.32; Lyase; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Mitochondrial; Kinase, other; Carbohydrate Metabolism - pyruvate

Chromosomal Location of Human Ortholog: 14q11.2

Cellular Component: mitochondrial matrix; mitochondrion

Molecular Function: GTP binding; metal ion binding; phosphoenolpyruvate carboxykinase (GTP) activity; phosphoenolpyruvate carboxykinase activity; protein binding

Biological Process: carbohydrate metabolic process; gluconeogenesis; glucose metabolic process

Disease: Phosphoenolpyruvate Carboxykinase Deficiency, Mitochondrial