Rabbit anti-Human, Mouse AP2M1 Polyclonal Antibody | anti-AP2M1 antibody

AP2M1, CT (AP2M1, CLAPM1, KIAA0109, AP-2 complex subunit mu, AP-2 mu chain, Adapter-related protein complex 2 mu subunit, Adaptin-mu2, Adaptor protein complex AP-2 subunit mu, Clathrin assembly protein complex 2 medium chain, Clathrin coat assembly protei

Cat. Num. AAA6274082

• Gene Names: AP2M1; mu2; AP50; CLAPM1
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: AP2M1; Polyclonal Antibody; CT (AP2M1; CLAPM1; KIAA0109; AP-2 complex subunit mu; AP-2 mu chain; Adapter-related protein complex 2 mu subunit; Adaptin-mu2; Adaptor protein complex AP-2 subunit mu; Clathrin assembly protein complex 2 medium chain; Clathrin coat assembly protei; Clathrin coat assembly protein AP50; Clathrin coat-associated protein AP50; HA2 50kD subunit; Plasma membrane adaptor AP-2 50kD protein; anti-AP2M1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-AP2M1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: AP2M1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 386-415 amino acids from the C-terminal region of human AP2M1.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(AP2M1 Antibody (C-term) western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the AP2M1 antibody detected the AP2M1 protein (arrow).)

Flow Cytometry (FC/FACS)

(AP2M1 Antibody (C-term) flow cytometric analysis of Neuro-2a cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-AP2M1 antibody

This gene encodes a subunit of the heterotetrameric coat assembly protein complex 2 (AP2), which belongs to the adaptor complexes medium subunits family. The encoded protein is required for the activity of a vacuolar ATPase, which is responsible for proton pumping occurring in the acidification of endosomes and lysosomes. The encoded protein may also play an important role in regulating the intracellular trafficking and function of CTLA-4 protein. Two transcript variants encoding different isoforms have been found for this gene.

Product Categories/Family for anti-AP2M1 antibody

Antibodies; Clathrin

References

Kahlfeldt, N., et al. J. Biol. Chem. 285(4):2734-2749(2010). Boucrot, E., et al. PLoS ONE 5 (5), E10597 (2010): Yoshida, T., et al. Int. J. Mol. Med. 24(2):233-246(2009). Levecque, C., et al. Am. J. Physiol., Cell Physiol. 297 (1), C160-C168 (2009): Stanasila, L., et al. Mol. Pharmacol. 74(3):562-573(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 68799814
• NCBI GeneID: 1173
• NCBI Accession #: NP_001020376.1; NP_004059.2
• NCBI GenBank Nucleotide #: NM_001025205.1
• UniProt Accession #: Q96CW1; P20172; P53679; A6NE12; D3DNT1
• Molecular Weight: 435
• NCBI Official Full Name: AP-2 complex subunit mu isoform b
• NCBI Official Synonym Full Names: adaptor-related protein complex 2, mu 1 subunit
• NCBI Official Symbol: AP2M1
• NCBI Official Synonym Symbols: mu2; AP50; CLAPM1
• NCBI Protein Information: AP-2 complex subunit mu; adaptin-mu2; AP-2 mu chain; AP-2 mu 2 chain; HA2 50 kDA subunit; clathrin coat adaptor protein AP50; clathrin coat assembly protein AP50; clathrin coat-associated protein AP50; adaptor protein complex AP-2 subunit mu; clathrin ada
• UniProt Protein Name: AP-2 complex subunit mu
• UniProt Gene Name: AP2M1
• UniProt Synonym Gene Names: CLAPM1; KIAA0109
• UniProt Entry Name: AP2M1_HUMAN

Uniprot Description

AP2M1: a protein of the adaptor complexes medium subunit family. A subunit of the heterotetrameric coat assembly protein complex 2 (AP2) which links clathrin to receptors in coated vesicles. Interacts with the cytoplasmic tails of membrane proteins and polyphosphoinositide-containing lipids. Is not required for clathrin-coated vesicle formation at the plasma membrane, but that it is one of several endocytic adaptors required for the uptake of certain cargo proteins. Is required for the activity of a vacuolar ATPase, which is responsible for proton pumping occurring in the acidification of endosomes and lysosomes. AP-2 is a heterotetramer composed of two large chains (alpha and beta), a medium chain (AP50) and a small chain (AP17).

Protein type: Vesicle; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 3q28

Cellular Component: mitochondrion; lysosomal membrane; plasma membrane; AP-2 adaptor complex; cytosol

Molecular Function: protein binding; low-density lipoprotein receptor binding; transporter activity; signal sequence binding; lipid binding

Biological Process: epidermal growth factor receptor signaling pathway; negative regulation of epidermal growth factor receptor signaling pathway; intracellular protein transport; axon guidance; synaptic transmission; viral reproduction; nerve growth factor receptor signaling pathway; ephrin receptor signaling pathway; antigen processing and presentation of exogenous peptide antigen via MHC class II; regulation of defense response to virus by virus