Western Blot (WB)
(Anti-CLEC4F Antibody (C-term) at 1:500 dilution + CCRF-CEM whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 66 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit anti-Human CLEC4F Polyclonal Antibody | anti-CLEC4F antibody
CLEC4F Antibody (C-term)
IHC-P-Leica~~1:250
Western Blot (WB)
(Anti-CLEC4F Antibody (C-term) at 1:500 dilution + CCRF-CEM whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 66 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(Anti-CLEC4F Antibody (C-term) at 1:500 dilution + CCRF-CEM whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 66 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot
(All lanes : Anti-CLEC4F Antibody (C-term) at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: U-2OS whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 66 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot
(All lanes : Anti-CLEC4F Antibody (C-term) at 1:2000 dilution Lane 1: Hela whole cell lysate
Lane 2: U-2OS whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 66 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of MBS9209164 on paraffin-embedded human skeletal muscle tissue was performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9.0). Samples were incubated with primary antibody(1:250) for 15min at room temperature. Leica Bond Polymer Refine Detection was used as the secondary antibody.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of MBS9209164 on paraffin-embedded human skeletal muscle tissue was performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9.0). Samples were incubated with primary antibody(1:250) for 15min at room temperature. Leica Bond Polymer Refine Detection was used as the secondary antibody.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of MBS9209164 on paraffin-embedded human liver tissue was performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:250) for 15min at room temperature. Leica Bond Polymer Refine Detection was used as the secondary antibody.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of MBS9209164 on paraffin-embedded human liver tissue was performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:250) for 15min at room temperature. Leica Bond Polymer Refine Detection was used as the secondary antibody.)
Hoyle, G.W., et al. J. Biol. Chem. 266(3):1850-1857(1991)
NCBI and Uniprot Product Information
Uniprot Description
CLEC4F: Receptor with an affinity for galactose and fucose. Could be involved in endocytosis.
Protein type: Membrane protein, integral; Extracellular matrix
Chromosomal Location of Human Ortholog: 2p13.3
Cellular Component: plasma membrane; integral to membrane
Molecular Function: galactose binding; glycolipid binding
Biological Process: endocytosis; NK T cell activation
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Product Notes
The CLEC4F clec4f (Catalog #AAA9209164) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 311-339. The CLEC4F Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CLEC4F can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P)-Leica. WB~~1:2000 IHC-P-Leica~~1:250. Researchers should empirically determine the suitability of the CLEC4F clec4f for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CLEC4F, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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