Rabbit anti-Human PCDHA6 Polyclonal Antibody | anti-PCDHA6 antibody

PCDHA6, CT (PCDHA6, CNRS2, Protocadherin alpha-6) (Azide free) (HRP)

Cat. Num. AAA6331499

• Gene Names: PCDHA6; CNR2; CNRN2; CNRS2; CRNR2; PCDH-ALPHA6
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PCDHA6; Polyclonal Antibody; CT (PCDHA6; CNRS2; Protocadherin alpha-6) (Azide free) (HRP); Protocadherin alpha-6; anti-PCDHA6 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-PCDHA6 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: PCDHA6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 772-800 amino acids from the C-terminal region of human PCDHA6.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(PCDHA6 Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the PCDHA6 antibody detected the PCDHA6 protein (arrow).)

Immunohistochemistry (IHC)

(PCDHA6 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PCDHA6 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(PCDHA6 Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-PCDHA6 antibody

This gene is a member of the protocadherin alpha gene cluster, one of three related gene clusters tandemly linked on chromosome five that demonstrate an unusual genomic organization similar to that of B-cell and T-cell receptor gene clusters. The alpha gene cluster is composed of 15 cadherin superfamily genes related to the mouse CNR genes and consists of 13 highly similar and 2 more distantly related coding sequences. The tandem array of 15 N-terminal exons, or variable exons, are followed by downstream C-terminal exons, or constant exons, which are shared by all genes in the cluster. The large, uninterrupted N-terminal exons each encode six cadherin ectodomains while the C-terminal exons encode the cytoplasmic domain. These neural cadherin-like cell adhesion proteins are integral plasma membrane proteins that most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been observed and additional variants have been suggested but their full-length nature has yet to be determined.

Product Categories/Family for anti-PCDHA6 antibody

Antibodies; Cadherin

References

Wu, Q., et al. Genome Res. 11(3):389-404(2001). Nollet, F., et al. J. Mol. Biol. 299(3):551-572(2000). Yagi, T., et al. Genes Dev. 14(10):1169-1180(2000). Wu, Q., et al. Proc. Natl. Acad. Sci. U.S.A. 97(7):3124-3129(2000). Sugino, H., et al. Genomics 63(1):75-87(2000).

NCBI and Uniprot Product Information

• NCBI GI #: 14165394
• NCBI GeneID: 56142
• NCBI Accession #: NP_114037.1
• NCBI GenBank Nucleotide #: NM_031849.2
• UniProt Accession #: Q9UN73; O75283; Q9NRT8
• Molecular Weight: 102,716 Da
• NCBI Official Full Name: protocadherin alpha-6 isoform 3
• NCBI Official Synonym Full Names: protocadherin alpha 6
• NCBI Official Symbol: PCDHA6
• NCBI Official Synonym Symbols: CNR2; CNRN2; CNRS2; CRNR2; PCDH-ALPHA6
• NCBI Protein Information: protocadherin alpha-6; PCDH-alpha-6; KIAA0345-like 8
• UniProt Protein Name: Protocadherin alpha-6
• Protein Family: Protocadherin
• UniProt Gene Name: PCDHA6
• UniProt Synonym Gene Names: CNRS2; PCDH-alpha-6
• UniProt Entry Name: PCDA6_HUMAN

NCBI Description

This gene is a member of the protocadherin alpha gene cluster, one of three related gene clusters tandemly linked on chromosome five that demonstrate an unusual genomic organization similar to that of B-cell and T-cell receptor gene clusters. The alpha gene cluster is composed of 15 cadherin superfamily genes related to the mouse CNR genes and consists of 13 highly similar and 2 more distantly related coding sequences. The tandem array of 15 N-terminal exons, or variable exons, are followed by downstream C-terminal exons, or constant exons, which are shared by all genes in the cluster. The large, uninterrupted N-terminal exons each encode six cadherin ectodomains while the C-terminal exons encode the cytoplasmic domain. These neural cadherin-like cell adhesion proteins are integral plasma membrane proteins that most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been observed and additional variants have been suggested but their full-length nature has yet to be determined. [provided by RefSeq, Jul 2008]