Rabbit anti-Human PCAF Polyclonal Antibody | anti-PCAF antibody

PCAF, CT (KAT2B, PCAF, Histone acetyltransferase KAT2B, Histone acetyltransferase PCAF, Lysine acetyltransferase 2B, P300/CBP-associated factor) (PE)

Cat. Num. AAA6331296

• Gene Names: KAT2B; P; CAF; PCAF; P/CAF
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Synonyms: PCAF; Polyclonal Antibody; CT (KAT2B; Histone acetyltransferase KAT2B; Histone acetyltransferase PCAF; Lysine acetyltransferase 2B; P300/CBP-associated factor) (PE); KAT2B; P300/CBP-associated factor; anti-PCAF antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-PCAF antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:50-100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: PCAF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 802-832 amino acids from the C-terminal region of human PCAF.
• Conjugate: PE
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(PCAF Antibody (C-term) western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the PCAF antibody detected the PCAF protein (arrow).)

Related Product Information for anti-PCAF antibody

CBP and p300 are large nuclear proteins that bind to many sequence-specific factors involved in cell growth and/or differentiation, including c-jun and the adenoviral oncoprotein E1A. The protein encoded by this gene associates with p300/CBP. It has in vitro and in vivo binding activity with CBP and p300, and competes with E1A for binding sites in p300/CBP. It has histone acetyl transferase activity with core histones and nucleosome core particles, indicating that this protein plays a direct role in transcriptional regulation.

Product Categories/Family for anti-PCAF antibody

Antibodies; Enzymes; Acetyltransferase

References

Perez, R.E., et al. J. Cell. Physiol. 225(2):394-405(2010). Mooney, S.M., et al. J. Biol. Chem. 285(40):30443-30452(2010). Aoyama, T., et al. J. Biol. Chem. 285(39):29842-29850(2010). Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Shimahara, A., et al. J. Biol. Chem. 285(22):16967-16977(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 40805843
• NCBI GeneID: 8850
• NCBI Accession #: NP_003875.3
• NCBI GenBank Nucleotide #: NM_003884.4
• UniProt Accession #: Q92831; Q6NSK1
• Molecular Weight: 93,013 Da
• NCBI Official Full Name: histone acetyltransferase KAT2B
• NCBI Official Synonym Full Names: K(lysine) acetyltransferase 2B
• NCBI Official Symbol: KAT2B
• NCBI Official Synonym Symbols: P; CAF; PCAF; P/CAF
• NCBI Protein Information: histone acetyltransferase KAT2B; histone acetylase PCAF; CREBBP-associated factor; p300/CBP-associated factor; lysine acetyltransferase 2B; histone acetyltransferase PCAF
• UniProt Protein Name: Histone acetyltransferase KAT2B
• UniProt Gene Name: KAT2B
• UniProt Synonym Gene Names: PCAF; Histone acetylase PCAF; P/CAF
• UniProt Entry Name: KAT2B_HUMAN

NCBI Description

CBP and p300 are large nuclear proteins that bind to many sequence-specific factors involved in cell growth and/or differentiation, including c-jun and the adenoviral oncoprotein E1A. The protein encoded by this gene associates with p300/CBP. It has in vitro and in vivo binding activity with CBP and p300, and competes with E1A for binding sites in p300/CBP. It has histone acetyl transferase activity with core histones and nucleosome core particles, indicating that this protein plays a direct role in transcriptional regulation. [provided by RefSeq, Jul 2008]

Uniprot Description

PCAF: Functions as a histone acetyltransferase (HAT) to promote transcriptional activation. Has significant histone acetyltransferase activity with core histones (H3 and H4), and also with nucleosome core particles. Inhibits cell-cycle progression and counteracts the mitogenic activity of the adenoviral oncoprotein E1A. In case of HIV-1 infection, it is recruited by the viral protein Tat. Regulates Tat's transactivating activity and may help inducing chromatin remodeling of proviral genes. Interacts with SIRT1. Interacts (unsumoylated form) with NR2C1; the interaction promotes transactivation activity. Interacts with EP300, CREBBP and DDX17. Interacts with NCOA1 and NCOA3. Component of a large chromatin remodeling complex, at least composed of MYSM1, KAT2B/PCAF, RBM10 and KIF11/TRIP5. Interacts with NR2C2 (hypophosphorylated and unsumoylated form); the interaction promotes the transactivation activity of NR2C2. Binds to HTLV-1 Tax. Interacts with and acetylates HIV-1 Tat. Interacts with KLF1; the interaction does not acetylate KLF1 and there is no enhancement of its transactivational activity. Interacts with NFE4. Interacts with MECOM. Interacts with E2F1; the interaction acetylates E2F1 augmenting its DNA-binding and transcriptional activity. Ubiquitously expressed but most abundant in heart and skeletal muscle. Belongs to the GCN5 family.

Protein type: Acetyltransferase; Nuclear receptor co-regulator; EC 2.3.1.48

Chromosomal Location of Human Ortholog: 3p24

Cellular Component: kinetochore; nucleoplasm; I band; PCAF complex; actomyosin; nucleus; A band

Molecular Function: lysine N-acetyltransferase activity; cyclin-dependent protein kinase inhibitor activity; protein binding; histone acetyltransferase activity; histone deacetylase binding; transcription coactivator activity; acetyltransferase activity; protein complex binding; transcription cofactor activity; transcription factor binding; protein kinase binding

Biological Process: transcription initiation from RNA polymerase II promoter; Notch signaling pathway; establishment and/or maintenance of chromatin architecture; internal peptidyl-lysine acetylation; viral reproduction; negative regulation of cyclin-dependent protein kinase activity; rhythmic process; transcription from RNA polymerase I promoter; chromatin remodeling; negative regulation of cell proliferation; protein amino acid acetylation; cellular response to insulin stimulus; gene expression; positive regulation of transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase I promoter; cell cycle arrest; peptidyl-lysine acetylation; N-terminal peptidyl-lysine acetylation