Western Blot (WB)
(All lanes : Anti-PBRM1 Antibody (C-term) at 1:2000 dilutionLane 1: F9 whole cell lysatesLane 2: NCCIT whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 193 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit anti-Human, mouse PBRM1 Polyclonal Antibody | anti-PBRM1 antibody
PBRM1 Antibody (C-term)
Western Blot (WB)
(All lanes : Anti-PBRM1 Antibody (C-term) at 1:2000 dilutionLane 1: F9 whole cell lysatesLane 2: NCCIT whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 193 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(All lanes : Anti-PBRM1 Antibody (C-term) at 1:2000 dilutionLane 1: F9 whole cell lysatesLane 2: NCCIT whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 193 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Horikawa I.,et al.DNA Seq. 13:211-215(2002).
Sekine I.,et al.Oncogene 24:2735-2738(2005).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Lemon B.,et al.Nature 414:924-928(2001).
NCBI and Uniprot Product Information
NCBI Description
This locus encodes a subunit of ATP-dependent chromatin-remodeling complexes. The encoded protein has been identified as in integral component of complexes necessary for ligand-dependent transcriptional activation by nuclear hormone receptors. Mutations at this locus have been associated with primary clear cell renal cell carcinoma. [provided by RefSeq, Feb 2012]
Uniprot Description
polybromo 1: Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Acts as a negative regulator of cell proliferation. Defects in PBRM1 are a cause of renal cell carcinoma (RCC). It is a heterogeneous group of sporadic or hereditary carcinoma derived from cells of the proximal renal tubular epithelium. It is subclassified into clear cell renal carcinoma (non-papillary carcinoma), papillary renal cell carcinoma, chromophobe renal cell carcinoma, collecting duct carcinoma with medullary carcinoma of the kidney, and unclassified renal cell carcinoma. 9 isoforms of the human protein are produced by alternative splicing.
Protein type: Tumor suppressor; DNA-binding
Chromosomal Location of Human Ortholog: 3p21
Cellular Component: kinetochore; nuclear chromosome
Molecular Function: protein binding; DNA binding; chromatin binding
Biological Process: chromatin remodeling; negative regulation of cell proliferation; mitosis; transcription, DNA-dependent; regulation of transcription, DNA-dependent; heart development; placenta development
Research Articles on PBRM1
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Product Notes
The PBRM1 pbrm1 (Catalog #AAA9204951) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PBRM1 Antibody (C-term) reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PBRM1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:2000. Researchers should empirically determine the suitability of the PBRM1 pbrm1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PBRM1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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