Rabbit PAX8 Polyclonal Antibody | anti-PAX8 antibody

PAX8 polyclonal antibody

Cat. Num. AAA3014587

• Reactivity: Human, Rat, Mouse
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. The purity is >95% (by SDS-PAGE).
• Synonyms: PAX8; Polyclonal Antibody; PAX8 polyclonal antibody; Myc proto-oncogene protein; Class E basic helix-loop-helix protein 39; bHLHe39; MYC; anti-PAX8 antibody

• Host: Rabbit
• Reactivity: Human, Rat, Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: PAX8 polyclonal antibody detects endogenous levels of PAX8 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. The purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.4.

• Applicable Applications for anti-PAX8 antibody: Western Blot (WB)
• Application Notes: WB: 1:500~1:1000
• Immunogen: Synthetic peptide, corresponding to Human PAX8.
• Preparation and Storage: Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze-thaw cycles.

Western Blot (WB)

(Western blot (WB) analysis of PXN polyclonal antibody at 1:500 dilutionLane1:A2780 whole cell lysate(40ug)Lane2:MCF-7 whole cell lysate(40ug)Lane3:HEK293T whole cell lysate(40ug)Lane4:HUT78 whole cell lysate(40ug)Lane5:SK-OVCAR3 whole cell lysate(40ug))

Related Product Information for anti-PAX8 antibody

Paired box (PAX) proteins are a family of transcription factors that play important and diverse roles in animal development. Nine PAX proteins (PAX1-9) have been described in humans and other mammals. They are defined by the presence of an amino-terminal "paired" domain, consisting of two helix-turn-helix motifs, with DNA binding activity. PAX proteins are classified into four structurally distinct subgroups (I-IV) based on the absence or presence of a carboxy-terminal homeodomain and a central octapeptide region. Subgroup I (PAX1 and 9) contains the octapeptide but lacks the homeodomain; subgroup II (PAX2, 5, and 8) contains the octapeptide and a truncated homeodomain; subgroup III (PAX3 and 7) contains the octapeptide and a complete homeodomain; and subgroup IV (PAX4 and 6) contains a complete homeodomain but lacks the octapeptide region. PAX proteins play critically important roles in development by regulating transcriptional networks responsible for embryonic patterning and organogenesis; a subset of PAX proteins also maintain functional importance during postnatal development. Research studies have implicated genetic mutations that result in aberrant expression of PAX genes in a number of cancer subtypes, with members of subgroups II and III identified as potential mediators of tumor progression.PAX8 is involved in the development of thyroid follicular cells and the expression of thyroid-specific genes (5). Investigators have associated mutations in the PAX8 gene with thyroid dysgenesis, thyroid follicular carcinomas, and atypical follicular thyroid adenomas (6,7).

NCBI and Uniprot Product Information

• NCBI GI #: 215273928
• NCBI GeneID: 7849
• UniProt Accession #: Q06710; Q09155; Q16337; Q16338; Q16339; Q4ZG35; Q96J49
• Molecular Weight: 295
• NCBI Official Full Name: Paired box protein Pax-8
• NCBI Official Synonym Full Names: paired box 8
• NCBI Official Symbol: PAX8
• NCBI Protein Information: paired box protein Pax-8; paired domain gene 8
• UniProt Protein Name: Paired box protein Pax-8
• Protein Family: Paired box protein
• UniProt Gene Name: PAX8
• UniProt Entry Name: PAX8_HUMAN

NCBI Description

This gene encodes a member of the paired box (PAX) family of transcription factors. Members of this gene family typically encode proteins that contain a paired box domain, an octapeptide, and a paired-type homeodomain. This nuclear protein is involved in thyroid follicular cell development and expression of thyroid-specific genes. Mutations in this gene have been associated with thyroid dysgenesis, thyroid follicular carcinomas and atypical follicular thyroid adenomas. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Mar 2010]

Uniprot Description

PAX8: Transcription factor for the thyroid-specific expression of the genes exclusively expressed in the thyroid cell type, maintaining the functional differentiation of such cells. Interacts with WWTR1. Expressed in the excretory system, thyroid gland and Wilms tumors. 5 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 2q13

Cellular Component: nucleoplasm; nucleus

Molecular Function: protein binding; DNA binding; transcription factor activity; thyroid-stimulating hormone receptor activity

Biological Process: regulation of apoptosis; anatomical structure morphogenesis; inner ear morphogenesis; central nervous system development; transcription, DNA-dependent; ureteric bud branching; positive regulation of transcription, DNA-dependent; thyroid gland development; positive regulation of transcription from RNA polymerase II promoter; mesonephros development; kidney development; pronephros development; urogenital system development

Disease: Hypothyroidism, Congenital, Nongoitrous, 2