Rabbit anti-Human, Mouse PAX7 Polyclonal Antibody | anti-PAX7 antibody

PAX7, CT (PAX7, HUP1, Paired box protein Pax-7, HuP1)

Cat. Num. AAA6005499

• Gene Names: PAX7; HUP1; RMS2; PAX7B
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: PAX7; Polyclonal Antibody; CT (PAX7; HUP1; Paired box protein Pax-7; HuP1); Anti -PAX7; anti-PAX7 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human, mouse
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-PAX7 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500; Immunohistochemistry: 1:50-100
• Immunogen: PAX7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 417-445 amino acids from the C-terminal region of human PAX7.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in mouse spleen tissue lysates (35ug/lane) using MBS6005499.This demonstrates theat MBS6005499 detected the PAX7 protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human spleen tissue using MBS6005499 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS6005499 for immunohistochemistry.)

Related Product Information for anti-PAX7 antibody

PAX7 is a member of the paired box (PAX) family of transcription factors. Members of this gene family typically contain a paired box domain, an octapeptide, and a paired-type homeodomain. These genes play critical roles during fetal development and cancer growth. The specific function of the paired box 7 gene is unknown but speculated to involve tumor suppression since fusion of this gene with a forkhead domain family member has been associated with alveolar rhabdomyosarcoma. Three transcript variants encoding different isoforms have been found for this gene.

Product Categories/Family for anti-PAX7 antibody

Antibodies; Abs to Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 2570015
• NCBI GeneID: 5081
• UniProt Accession #: P23759; Q0VA99; Q2PJS5; E9PFV9
• Molecular Weight: 56,896 Da
• NCBI Official Full Name: PAX7
• NCBI Official Synonym Full Names: paired box 7
• NCBI Official Symbol: PAX7
• NCBI Official Synonym Symbols: HUP1; RMS2; PAX7B
• NCBI Protein Information: paired box protein Pax-7; paired domain gene 7; paired box homeotic gene 7; PAX7 transcriptional factor
• UniProt Protein Name: Paired box protein Pax-7
• Protein Family: Paired box protein
• UniProt Gene Name: PAX7
• UniProt Synonym Gene Names: HUP1
• UniProt Entry Name: PAX7_HUMAN

NCBI Description

This gene is a member of the paired box (PAX) family of transcription factors. Members of this gene family typically contain a paired box domain, an octapeptide, and a paired-type homeodomain. These genes play critical roles during fetal development and cancer growth. The specific function of the paired box 7 gene is unknown but speculated to involve tumor suppression since fusion of this gene with a forkhead domain family member has been associated with alveolar rhabdomyosarcoma. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2008]

Uniprot Description

PAX7: Probable transcription factor. May have a role in myogenesis. Defects in PAX7 are a cause of rhabdomyosarcoma type 2 (RMS2). It is a form of rhabdomyosarcoma, a highly malignant tumor of striated muscle derived from primitive mesenchimal cells and exhibiting differentiation along rhabdomyoblastic lines. Rhabdomyosarcoma is one of the most frequently occurring soft tissue sarcomas and the most common in children. It occurs in four forms: alveolar, pleomorphic, embryonal and botryoidal rhabdomyosarcomas. A chromosomal aberration involving PAX7 is found in rhabdomyosarcoma. Translocation t(1;13)(p36;q14) with FOXO1. The resulting protein is a transcriptional activator. Belongs to the paired homeobox family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Oncoprotein; Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 1p36.13

Cellular Component: nucleus

Molecular Function: sequence-specific DNA binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; anatomical structure morphogenesis; positive regulation of histone methylation; embryonic skeletal development; satellite cell commitment; neuron fate commitment; skeletal muscle regeneration; chromatin remodeling; regulation of protein binding; cartilage development; regulation of cell fate commitment; spinal cord association neuron differentiation; positive regulation of transcription from RNA polymerase II promoter; dorsoventral neural tube patterning; negative regulation of apoptosis

Disease: Rhabdomyosarcoma 2