Rabbit anti-Human, Mouse PAX7 Polyclonal Antibody | anti-PAX7 antibody

Rabbit Polyclonal to Human PAX7

Cat. Num. AAA248402

• Gene Names: PAX7; HUP1; RMS2; PAX7B
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot
• Purity: Immunoaffinity Purified
• Synonyms: PAX7; Polyclonal Antibody; Rabbit Polyclonal to Human PAX7; Anti-PAX7 Antibody (aa410-437) IHC-plus; HUP1; Paired box 7; Paired box gene 7; Paired box homeotic gene 7; Paired box protein Pax-7; RMS2; Paired domain gene 7; PAX7 transcriptional factor; PAX7B; Human PAX7; anti-PAX7 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Specificity: Human PAX7
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, 0.09% sodium azide
• Sequence Length: 520

• Applicable Applications for anti-PAX7 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
• Application Notes: IHC-P (5 ug/ml), WB (1:1000); Optimal dilution to be determined by the researcher.
• Target Species: Human
• Immunogen Description: KLH-conjugated synthetic peptide from C-terminal region of human PAX7.
• Immunogen Type: Synthetic peptide
• Immunogen: PAX7 antibody was raised against kLH-conjugated synthetic peptide from C-terminal region of human PAX7.
• Epitope: aa410-437
• Specificity Note: This PAX7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 410-437 amino acids from the C-terminal region of human PAX7.
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Immunohistochemistry (IHC)

(Anti-PAX7 antibody IHC staining of human skin, epidermis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Western Blot (WB)

(PAX7 Antibody (C-term) Western blot of mouse spleen tissue lysates (35 ug/lane). This demonstrates the PAX7 antibody detected the PAX7 protein (arrow).)

Related Product Information for anti-PAX7 antibody

This gene is a member of the paired box (PAX) family of transcription factors. Members of this gene family typically contain a paired box domain, an octapeptide, and a paired-type homeodomain. These genes play critical roles during fetal development and cancer growth. The specific function of the paired box gene 7 is unknown but speculated to involve tumor suppression since fusion of this gene with a forkhead domain family member has been associated with alveolar rhabdomyosarcoma.

NCBI and Uniprot Product Information

• NCBI GI #: 4505619
• NCBI GeneID: 5081
• NCBI Accession #: NP_002575.1
• NCBI GenBank Nucleotide #: NM_002584.2
• UniProt Accession #: P23759; Q0VA99; Q2PJS5; E9PFV9
• NCBI Official Full Name: paired box protein Pax-7 isoform 1
• NCBI Official Synonym Full Names: paired box 7
• NCBI Official Symbol: PAX7
• NCBI Official Synonym Symbols: HUP1; RMS2; PAX7B
• NCBI Protein Information: paired box protein Pax-7; paired domain gene 7; paired box homeotic gene 7; PAX7 transcriptional factor
• UniProt Protein Name: Paired box protein Pax-7
• Protein Family: Paired box protein
• UniProt Gene Name: PAX7
• UniProt Synonym Gene Names: HUP1
• UniProt Entry Name: PAX7_HUMAN

NCBI Description

This gene is a member of the paired box (PAX) family of transcription factors. Members of this gene family typically contain a paired box domain, an octapeptide, and a paired-type homeodomain. These genes play critical roles during fetal development and cancer growth. The specific function of the paired box 7 gene is unknown but speculated to involve tumor suppression since fusion of this gene with a forkhead domain family member has been associated with alveolar rhabdomyosarcoma. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2008]

Uniprot Description

PAX7: Probable transcription factor. May have a role in myogenesis. Defects in PAX7 are a cause of rhabdomyosarcoma type 2 (RMS2). It is a form of rhabdomyosarcoma, a highly malignant tumor of striated muscle derived from primitive mesenchimal cells and exhibiting differentiation along rhabdomyoblastic lines. Rhabdomyosarcoma is one of the most frequently occurring soft tissue sarcomas and the most common in children. It occurs in four forms: alveolar, pleomorphic, embryonal and botryoidal rhabdomyosarcomas. A chromosomal aberration involving PAX7 is found in rhabdomyosarcoma. Translocation t(1;13)(p36;q14) with FOXO1. The resulting protein is a transcriptional activator. Belongs to the paired homeobox family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Oncoprotein; Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 1p36.13

Cellular Component: nucleus

Molecular Function: sequence-specific DNA binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; anatomical structure morphogenesis; positive regulation of histone methylation; embryonic skeletal development; satellite cell commitment; neuron fate commitment; skeletal muscle regeneration; chromatin remodeling; regulation of protein binding; cartilage development; regulation of cell fate commitment; spinal cord association neuron differentiation; positive regulation of transcription from RNA polymerase II promoter; dorsoventral neural tube patterning; negative regulation of apoptosis

Disease: Rhabdomyosarcoma 2