Rabbit anti-Human PAX3 Polyclonal Antibody | anti-PAX3 antibody

PAX3, NT (Paired Box Protein Pax-3, HUP2) (PE)

Cat. Num. AAA6492236

• Gene Names: PAX3; WS1; WS3; CDHS; HUP2
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: PAX3; Polyclonal Antibody; NT (Paired Box Protein Pax-3; HUP2) (PE); anti-PAX3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PAX3.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-PAX3 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-1:500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human PAX3 (KLH).
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(PAX3 Antibody (N-term) western blot analysis in A431 cell line lysates (35ug/lane).This demonstrates the PAX3 antibody detected the PAX3 protein (arrow).)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human skin reacted with PAX3 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of K562 cells using PAX3 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-PAX3 antibody

Antibodies; Transcription Factors

References

1. Boutet, S.C., et.al., Cell 130(2), 349-362 (2007).

NCBI and Uniprot Product Information

• NCBI GI #: 31563340
• NCBI GeneID: 5077
• NCBI Accession #: NP_852122
• NCBI GenBank Nucleotide #: NM_181457
• UniProt Accession #: P23760
• Molecular Weight: 53kDa
• NCBI Official Full Name: paired box protein Pax-3 isoform PAX3
• NCBI Official Synonym Full Names: paired box 3
• NCBI Official Symbol: PAX3
• NCBI Official Synonym Symbols: WS1; WS3; CDHS; HUP2
• NCBI Protein Information: paired box protein Pax-3
• UniProt Protein Name: Paired box protein Pax-3
• Protein Family: Paired box protein
• UniProt Gene Name: PAX3
• UniProt Synonym Gene Names: HUP2
• UniProt Entry Name: PAX3_HUMAN

NCBI Description

This gene is a member of the paired box (PAX) family of transcription factors. Members of the PAX family typically contain a paired box domain and a paired-type homeodomain. These genes play critical roles during fetal development. Mutations in paired box gene 3 are associated with Waardenburg syndrome, craniofacial-deafness-hand syndrome, and alveolar rhabdomyosarcoma. The translocation t(2;13)(q35;q14), which represents a fusion between PAX3 and the forkhead gene, is a frequent finding in alveolar rhabdomyosarcoma. Alternative splicing results in transcripts encoding isoforms with different C-termini. [provided by RefSeq, Jul 2008]

Uniprot Description

PAX3: Probable transcription factor associated with development of alveolar rhabdomyosarcoma. Can bind to DNA as a heterodimer with PAX7. Interacts with DAXX. Belongs to the paired homeobox family. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; Oncoprotein; DNA-binding

Chromosomal Location of Human Ortholog: 2q35

Cellular Component: nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; sequence-specific DNA binding; chromatin binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; muscle development; apoptosis; heart development; positive regulation of transcription, DNA-dependent; pigmentation during development; neuron fate commitment; negative regulation of transcription from RNA polymerase II promoter; cell proliferation; organ morphogenesis; sensory perception of sound; positive regulation of cell proliferation; neural tube closure; spinal cord association neuron differentiation; positive regulation of transcription from RNA polymerase II promoter; regulation of somitogenesis; neural crest cell migration

Disease: Waardenburg Syndrome, Type 3; Craniofacial-deafness-hand Syndrome; Rhabdomyosarcoma 2; Waardenburg Syndrome, Type 1