Rabbit anti-Rat LOX-1 Polyclonal Antibody | anti-OLR1 antibody

Anti-LOX-1 Antibody, Rabbit Polyclonal

Cat. Num. AAA8103307

• Gene Names: Olr1; LOX-1; Oldr1; Oldlr1
• Reactivity: Rat
• Applications: Immunohistochemistry
• Purity: Protein A & Antigen Affinity
• Synonyms: LOX-1; Polyclonal Antibody; Anti-LOX-1 Antibody; Rabbit Polyclonal; OLR1/LOX1 Antibody; Rabbit PAb; Antigen Affinity Purified; oxidized low density lipoprotein (lectin-like) receptor 1; Anti-Lox1 Antibody; Anti-Oldlr1 Antibody; Anti-OLR1 Antibody; anti-OLR1 antibody

• Host: Rabbit
• Reactivity: Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Rat OLR1/LOX1
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-OLR1 antibody: Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: IHC-P: 1:500-1:2000
• Immunogen: A synthetic peptide corresponding to the center region of the Rat OLR1/LOX1
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Rat OLR1/LOX1, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of rat OLR1 in rat kidney with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)

Related Product Information for anti-OLR1 antibody

Oxidized low-density lipoprotein receptor 1 (Ox-LDL receptor 1 or OLR1), also known as lectin-type oxidized LDL receptor 1 (LOX1), is a receptor protein that belongs to the C-type lectin superfamily. LOX1 is a multi-ligand receptor originally identified as the endothelial oxidized LDL receptor. OLR1/LOX1 was isolated from an aortic endothelial cell, and recently it has been discovered in macrophages and vascular smooth muscle cells in artery vessels. The expression of LOX1 is induced by inflammatory stimuli and oxidative stimuli. This protein binds, internalizes, and degrades oxidized low-density lipoprotein. LOX1 may play an important role in the progression of vulnerable carotid plaque and might regulate vulnerable plaque formation in cooperation with MMPs and TIMP-2. In clinical, LOX1 is thought to be involved in the development of atherosclerotic lesions.

References

Hinagata J, et al. (2006) Oxidized LDL receptor LOX-1 is involved in neointimal hyperplasia after balloon arterial injury in a rat model. Cardiovasc Res. 69 (1): 263-71.Melan MA, et al. (1994) The LOX1 Gene of Arabidopsis Is Temporally and Spatially Regulated in Germinating Seedlings. Plant Physiol. 105 (1): 385-93.Saito A, et al. (2010) Relationship between lectin-like oxidized low-density lipoprotein receptor 1 expression and preoperative echogenic findings of vulnerable carotid plaque. Acta Neurochir (Wien). 152 (4): 589-95.

NCBI and Uniprot Product Information

• NCBI GI #: 4008187
• NCBI GeneID: 140914
• UniProt Accession #: O70156
• Molecular Weight: 41,890 Da
• NCBI Official Full Name: lectin-like oxidized low-density lipoprotein receptor
• NCBI Official Synonym Full Names: oxidized low density lipoprotein (lectin-like) receptor 1
• NCBI Official Symbol: Olr1
• NCBI Official Synonym Symbols: LOX-1; Oldr1; Oldlr1
• NCBI Protein Information: oxidized low-density lipoprotein receptor 1; ox-LDL receptor 1; lectin-like oxLDL receptor 1; lectin-like oxidized LDL receptor 1; lectin-type oxidized LDL receptor 1; lectin-like oxidized low-density lipoprotein receptor; Lectin-like oxidized low-density
• UniProt Protein Name: Oxidized low-density lipoprotein receptor 1
• Protein Family: Oxidized low-density lipoprotein receptor
• UniProt Gene Name: Olr1
• UniProt Synonym Gene Names: Lox1; Oldlr1; Ox-LDL receptor 1; LOX-1
• UniProt Entry Name: OLR1_RAT

NCBI Description

receptor for oxidized low-density lipoprotein that may be involved in pathogenesis of hypertension as well as atherosclerosis [RGD, Feb 2006]

Uniprot Description

Function: Receptor that mediates the recognition, internalization and degradation of oxidatively modified low density lipoprotein (oxLDL) by vascular endothelial cells. OxLDL is a marker of atherosclerosis that induces vascular endothelial cell activation and dysfunction, resulting in pro-inflammatory responses, pro-oxidative conditions and apoptosis. Its association with oxLDL induces the activation of NF-kappa-B through an increased production of intracellular reactive oxygen and a variety of pro-atherogenic cellular responses including a reduction of nitric oxide (NO) release, monocyte adhesion and apoptosis. In addition to binding oxLDL, it acts as a receptor for the HSP70 protein involved in antigen cross-presentation to naive T-cells in dendritic cells, thereby participating in cell-mediated antigen cross-presentation. Also involved in inflammatory process, by acting as a leukocyte-adhesion molecule at the vascular interface in endotoxin-induced inflammation. Also acts as a receptor for advanced glycation end (AGE) products, activated platelets, monocytes, apoptotic cells and both Gram-negative and Gram-positive bacteria. Ref.2 Ref.4

Subunit structure: Homodimer; disulfide-linked. May form a hexamer composed of 3 homodimers. Interacts with HSP70

By similarity.

Subcellular location: Cell membrane; Lipid-anchor

By similarity. Cell membrane; Single-pass type II membrane protein

By similarity. Membrane raft

By similarity. Secreted

By similarity. Note: A secreted form also exists. Localization to membrane rafts requires palmitoylation

By similarity.

Tissue specificity: Predominantly expressed in lung and at lower level in kidney. Expressed in macrophages but not in vascular smooth muscle cells. Ref.1

Induction: By hypertension. Up-regulated by shear stress, lipopolysaccharide and TNF-alpha in cultured vascular endothelial cells. Ref.1 Ref.2

Domain: The cytoplasmic region is required for subcellular sorting on the cell surface

By similarity.The C-type lectin domain mediates the recognition and binding of oxLDL

By similarity.The Neck region contains 3 internal repeats that are only found in rodents.

Post-translational modification: N-glycosylated

By similarity.

Sequence similarities: Contains 1 C-type lectin domain.