Bovine Stromal Cell Derived Factor 2 ELISA Kit | SDF2 elisa kit

Bovine Stromal Cell Derived Factor 2 ELISA Kit

Cat. Num. AAA737568

• Reactivity: Bovine
• Synonyms: Stromal Cell Derived Factor 2; Bovine Stromal Cell Derived Factor 2 ELISA Kit; SDF2 elisa kit

• Reactivity: Bovine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1ng/mL.
• Intended Uses: This SDF2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine SDF2. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SDF2 elisa kit

Principle of the assay: SDF2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-SDF2 antibody and an SDF2-HRP conjugate. The assay sample and buffer are incubated together with SDF2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SDF2 concentration since SDF2 from samples and SDF2-HRP conjugate compete for the anti-SDF2 antibody binding site. Since the number of sites is limited, as more sites are occupied by SDF2 from the sample, fewer sites are left to bind SDF2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SDF2 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for SDF2 elisa kit

Immunology

NCBI and Uniprot Product Information

• NCBI GI #: 14141195
• NCBI GeneID: 6388
• NCBI Accession #: NP_008854.2
• NCBI GenBank Nucleotide #: NM_006923.3
• Molecular Weight: 23,026 Da
• NCBI Official Full Name: stromal cell-derived factor 2
• NCBI Official Synonym Full Names: stromal cell-derived factor 2
• NCBI Official Symbol: SDF2
• NCBI Protein Information: stromal cell-derived factor 2; SDF-2
• UniProt Protein Name: Stromal cell-derived factor 2
• Protein Family: Stromal cell-derived factor
• UniProt Gene Name: SDF2
• UniProt Synonym Gene Names: SDF-2
• UniProt Entry Name: SDF2_HUMAN

NCBI Description

The protein encoded by this gene is believed to be a secretory protein. It has regions of similarity to hydrophilic segments of yeast mannosyltransferases. Its expression is ubiquitous and the gene appears to be relatively conserved among mammals. Alternate splicing results in both coding and non-coding variants. A pseudogene of this gene is located on chromosome 15. [provided by RefSeq, Dec 2011]

Uniprot Description

SDF2:

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 17q11.2

Cellular Component: extracellular space; membrane

Molecular Function: dolichyl-phosphate-mannose-protein mannosyltransferase activity

Biological Process: protein amino acid O-linked mannosylation; protein amino acid glycosylation