Western Blot (WB)
(Figure 1. Western blot analysis of PAX1 using anti-PAX1 antibody (MBS1753664).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: human placenta tissue lysatesLane 2: human Sw579 whole cell lysatesLane 3: human A431 whole cell lysatesLane 4: human U20S whole cell lysatesLane 5: rat thymus tissue lysatesLane 6: rat ovary tissue lytsatesLane 7: rat lung tissue lysatesLane 8: mouse thymus tissue lysatesLane 9: mouse ovary tissue lysatesLane 10: mouse lung tissue lysatesLane 11: mouse SP2/0 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-PAX1 antigen affinity purified polyclonal antibody (Catalog # MBS1753664) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for PAX1 at approximately 60KD. The expected band size for PAX1 is at 60KD. )
Rabbit PAX1 Polyclonal Antibody | anti-PAX1 antibody
Anti-PAX1 Antibody
FC/FACS/FCM: 1-3ug/1x106 cells|Human|
Direct ELISA: 0.1-0.5ug/ml|Human|
Western Blot (WB)
(Figure 1. Western blot analysis of PAX1 using anti-PAX1 antibody (MBS1753664).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: human placenta tissue lysatesLane 2: human Sw579 whole cell lysatesLane 3: human A431 whole cell lysatesLane 4: human U20S whole cell lysatesLane 5: rat thymus tissue lysatesLane 6: rat ovary tissue lytsatesLane 7: rat lung tissue lysatesLane 8: mouse thymus tissue lysatesLane 9: mouse ovary tissue lysatesLane 10: mouse lung tissue lysatesLane 11: mouse SP2/0 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-PAX1 antigen affinity purified polyclonal antibody (Catalog # MBS1753664) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for PAX1 at approximately 60KD. The expected band size for PAX1 is at 60KD. )
Western Blot (WB)
(Figure 1. Western blot analysis of PAX1 using anti-PAX1 antibody (MBS1753664).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: human placenta tissue lysatesLane 2: human Sw579 whole cell lysatesLane 3: human A431 whole cell lysatesLane 4: human U20S whole cell lysatesLane 5: rat thymus tissue lysatesLane 6: rat ovary tissue lytsatesLane 7: rat lung tissue lysatesLane 8: mouse thymus tissue lysatesLane 9: mouse ovary tissue lysatesLane 10: mouse lung tissue lysatesLane 11: mouse SP2/0 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-PAX1 antigen affinity purified polyclonal antibody (Catalog # MBS1753664) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for PAX1 at approximately 60KD. The expected band size for PAX1 is at 60KD. )
Flow Cytometry (FC/FACS)
(Figure 2. Flow Cytometry analysis of 293T cells using anti-PAX1 antibody (MBS1753664).Overlay histogram showing 293T cells stained with MBS1753664 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PAX1 Antibody (MBS1753664, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. )
Flow Cytometry (FC/FACS)
(Figure 2. Flow Cytometry analysis of 293T cells using anti-PAX1 antibody (MBS1753664).Overlay histogram showing 293T cells stained with MBS1753664 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PAX1 Antibody (MBS1753664, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. )
2. McGaughran, J. M., Oates, A., Donnai, D., Read, A. P., Tassabehji, M. Mutations in PAX1 may be associated with Klippel-Feil syndrome. Europ. J. Hum. Genet. 11: 468-474, 2003.
3. Pohl, E., Aykut, A., Beleggia, F., Karaca, E., Durmaz, B., Keupp, K., Arslan, E., Palamar, M., Yigit, G., Ozkinay, F., Wollnik, B. A hypofunctional PAX1 mutation causes autosomal recessively inherited otofaciocervical syndrome. Hum. Genet. 132: 1311-1320, 2013. Note: Erratum: Hum. Genet. 132: 1321 only, 2013.
NCBI and Uniprot Product Information
NCBI Description
This gene is a member of the paired box (PAX) family of transcription factors. Members of the PAX family typically contain a paired box domain and a paired-type homeodomain. These genes play critical roles during fetal development. This gene plays a role in pattern formation during embryogenesis and may be essential for development of the vertebral column. This gene is silenced by methylation in ovarian and cervical cancers and may be a tumor suppressor gene. Mutations in this gene are also associated with vertebral malformations. [provided by RefSeq, Mar 2012]
Uniprot Description
PAX1: This protein is a transcriptional activator. It may play a role in the formation of segmented structures of the embryo. May play an important role in the normal development of the vertebral column. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Transcription factor; DNA-binding
Chromosomal Location of Human Ortholog: 20p11.2
Cellular Component: nucleus
Molecular Function: DNA binding
Biological Process: transcription from RNA polymerase II promoter; cell proliferation; CD8-positive, alpha-beta T cell differentiation; somitogenesis; CD4-positive, alpha beta T cell differentiation; thymus development; positive regulation of transcription from RNA polymerase II promoter; parathyroid gland development; skeletal development
Disease: Otofaciocervical Syndrome 2
Research Articles on PAX1
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Product Notes
The PAX1 pax1 (Catalog #AAA1753664) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PAX1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PAX1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Flow Cytometry (FC/FACS/FCM), Direct ELISA (EIA). WB: 0.25-0.5ug/ml|Human, Mouse, Rat| FC/FACS/FCM: 1-3ug/1x106 cells|Human| Direct ELISA: 0.1-0.5ug/ml|Human|. Researchers should empirically determine the suitability of the PAX1 pax1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PAX1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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