Mouse MDC1 Monoclonal Antibody | anti-Mdc1 antibody

MDC1 Antibody: FITC

Cat. Num. AAA802152

• Gene Names: Mdc1; Nfbd1; AA413496; mKIAA0170; 6820401C03
• Reactivity: Mouse, Human, Chimpanzee, Bovine
• Applications: Immunofluorescence, Western Blot
• Synonyms: MDC1; Monoclonal Antibody; MDC1 Antibody: FITC; Nuclear factor with BRCT domains1; mediator of DNA damage checkpoint 1; anti-Mdc1 antibody

• Host: Mouse
• Reactivity: Mouse, Human, Chimpanzee, Bovine
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: P2B11
• Form/Format: Protein G Purified
• Concentration: 1mg/mL (varies by lot)
• Sequence Length: 1708

• Applicable Applications for anti-Mdc1 antibody: Immunofluorescence (IF), Western Blot (WB)
• Application Notes: 1:2000 (WB)
• Conjugate: FITC
• Storage Buffer: PBS pH7.4, 50% glycerol, 0.09% sodium azide
• Preparation and Storage: -20 degree C

Western Blot (WB)

(Western Blot analysis of Human Embryonic kidney epithelial cell line (HEK293T) lysate showing detection of 184 kDa MDC1 protein using Mouse Anti-MDC1 Monoclonal Antibody, Clone P2B11. Lane 1: MW ladder. Lane 2: 293Trap cell lysates. Load: 30 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-MDC1 Monoclonal Antibody at 1:1000 for 2 hours RT. Secondary Antibody: Goat Anti-Mouse HRP: IgG at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 184 kDa.)

Western Blot (WB)

(Western Blot analysis of Mouse Cortex and Cerebellum showing detection of 184 kDa MDC1 protein using Mouse Anti-MDC1 Monoclonal Antibody, Clone P2B11. Lane 1: MW ladder. Lane 2: Mouse Cortex. Lane 3: Mouse Cerebellum. Load: 10 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-MDC1 Monoclonal Antibody at 1:1000 for 2 hours RT. Secondary Antibody: Goat Anti-Mouse at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 184 kDa.)

Related Product Information for anti-Mdc1 antibody

Background Info: Detects ~184kDa. This antibody recognizes MDC1 at or around the N-terminus

Scientific Background: MDC1, mediator of DNA damage checkpoint protein 1, plays a role in checkpoint mediated cell cycle arrest in response to DNA damage, within S phase and G2/M. It is also thought to act as a scaffold protein during recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage that are marked by phosphorylation of histone H2A.X on S139.

Product Categories/Family for anti-Mdc1 antibody

Oxidative Stress

References

1. Lou Z., et al. (2004) J Biol Chem. 279(45): 46359-46362. 2. Luo K., Yuan J, Lou Z. (2011) J Biol Chem. 286(32): 28192-28199. 3. Strauss C., Halevy T., Macarov M., Argaman L., and Goldbery M. (2011) DNA Repair (Amst.). 10(8): 806-814. 4. Wilson K.A., et al. (2011) Mol Cancer Res. 9(6): 766-781.

NCBI and Uniprot Product Information

• NCBI GI #: 132626693
• NCBI GeneID: 240087
• NCBI Accession #: NP_001010833.2
• NCBI GenBank Nucleotide #: NM_001010833.2
• UniProt Accession #: Q5PSV9; Q52KG1; Q5U4D3; Q6ZQH7
• Molecular Weight: 184,670 Da
• NCBI Official Full Name: mediator of DNA damage checkpoint protein 1
• NCBI Official Synonym Full Names: mediator of DNA damage checkpoint 1
• NCBI Official Symbol: Mdc1
• NCBI Official Synonym Symbols: Nfbd1; AA413496; mKIAA0170; 6820401C03
• NCBI Protein Information: mediator of DNA damage checkpoint protein 1; mediation of DNA damage checkpoint 1
• UniProt Protein Name: Mediator of DNA damage checkpoint protein 1
• Protein Family: Mediator of DNA damage checkpoint protein
• UniProt Gene Name: Mdc1
• UniProt Synonym Gene Names: Kiaa0170
• UniProt Entry Name: MDC1_MOUSE

NCBI Description

The protein encoded by this gene contains an N-terminal forkhead domain, two BRCA1 C-terminal (BRCT) motifs and a central domain with 7 divergent copies of an approximately 41-amino acid sequence. The encoded protein is required to activate the intra-S phase and G2/M phase cell cycle checkpoints in response to DNA damage. This nuclear protein interacts with phosphorylated histone H2AX near sites of DNA double-strand breaks through its BRCT motifs, and facilitates recruitment of the ATM kinase and meiotic recombination 11 protein complex to DNA damage foci. Mice with mutations in this gene exhibit growth retardation, male infertility, immune defects, chromosome instability, DNA repair defects, and radiation sensitivity. [provided by RefSeq, Jul 2008]

Uniprot Description

MDC1: Required for checkpoint mediated cell cycle arrest in response to DNA damage within both the S phase and G2/M phases of the cell cycle. May serve as a scaffold for the recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage marked by 'Ser-139' phosphorylation of histone H2AFX. Also required for downstream events subsequent to the recruitment of these proteins. These include phosphorylation and activation of the ATM, CHEK1 and CHEK2 kinases, and stabilization of TP53 and apoptosis. ATM and CHEK2 may also be activated independently by a parallel pathway mediated by TP53BP1. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation

Cellular Component: nucleoplasm; focal adhesion; chromosome; nucleus

Molecular Function: protein C-terminus binding

Biological Process: cell cycle; DNA repair; response to DNA damage stimulus