Western Blot (WB)
(Western blot analysis of PASK (Phospho-Thr1165) using HeLa whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)
Rabbit PASK Polyclonal Antibody | anti-PASK antibody
Phospho-PASK (Thr1165) Antibody
Gene Names
PASK; STK37; PASKIN
Reactivity
Human, Mouse, RatPredicted Reactivity: Pig (100%), Zebrafish (88%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (88%), Dog (88%), Chicken (100%)
Applications
Western Blot, ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Synonyms
PASK; Polyclonal Antibody; Phospho-PASK (Thr1165) Antibody; DKFZP434O051; DKFZp686P2031; hPASK; KIAA0135; MGC93882; mKIAA0135; PAS domain containing serine/threonine kinase; PAS domain containing serine/threonine protein kinase; PAS domain-containing serine/threonine-protein kinase; PAS kinase; PAS serine/threonine kinase; PAS-kinase; Pask; PASK_HUMAN; PASKIN; STK 37; STK37; anti-PASK antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Zebrafish (88%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (88%), Dog (88%), Chicken (100%)
Predicted Reactivity: Pig (100%), Zebrafish (88%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (88%), Dog (88%), Chicken (100%)
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Phospho-PASK (Thr1165) Antibody detects endogenous levels of PASK only when phosphorylated at Thr1165.
Tissue Specificity: Ubiquitously expressed, with slightly higher expression in brain, prostate and testis. Reduced expression was found in placenta. Present in germ cells of testis and in the midpiece of sperm tails (at protein level).
Tissue Specificity: Ubiquitously expressed, with slightly higher expression in brain, prostate and testis. Reduced expression was found in placenta. Present in germ cells of testis and in the midpiece of sperm tails (at protein level).
Purity/Purification
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-PASK antibody
Western Blot (WB), Peptide ELISA (EIA)
Application Notes
WB: 1:1000-3000
Peptide ELISA: 1:20,000-1:40,000
Peptide ELISA: 1:20,000-1:40,000
Immunogen
A synthesized peptide derived from human PASK around the phosphorylation site of Thr1165.
Conjugation
Unconjugated
Fragment
Fab fragment
Post Translational Modifications
Autophosphorylated on Thr-1161 and Thr-1165. Autophosphorylation is activated by phospholipids.
Similarity
The protein kinase domain mediates binding to phosphatidylinositol.Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
Subcellular Location
Cytoplasm. Nucleus.
Note: Localizes in the nucleus of testis germ cells and in the midpiece of sperm tails.
Note: Localizes in the nucleus of testis germ cells and in the midpiece of sperm tails.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of PASK (Phospho-Thr1165) using HeLa whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)
Western Blot (WB)
(Western blot analysis of PASK (Phospho-Thr1165) using HeLa whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)
Related Product Information for anti-PASK antibody
Serine/threonine-protein kinase involved in energy homeostasis and protein translation. Phosphorylates EEF1A1, GYS1, PDX1 and RPS6. Probably plays a role under changing environmental conditions (oxygen, glucose, nutrition), rather than under standard conditions. Acts as a sensor involved in energy homeostasis: regulates glycogen synthase synthesis by mediating phosphorylation of GYS1, leading to GYS1 inactivation. May be involved in glucose-stimulated insulin production in pancreas and regulation of glucagon secretion by glucose in alpha cells; however such data require additional evidences. May play a role in regulation of protein translation by phosphorylating EEF1A1, leading to increase translation efficiency. May also participate to respiratory regulation.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
123,048 Da
NCBI Official Full Name
PAS domain-containing serine/threonine-protein kinase isoform 2
NCBI Official Synonym Full Names
PAS domain containing serine/threonine kinase
NCBI Official Symbol
PASK
NCBI Official Synonym Symbols
STK37; PASKIN
NCBI Protein Information
PAS domain-containing serine/threonine-protein kinase; per-arnt-sim (PAS) domain kinase
UniProt Protein Name
PAS domain-containing serine/threonine-protein kinase
Protein Family
UniProt Gene Name
PASK
UniProt Synonym Gene Names
KIAA0135; PAS-kinase; PASKIN; hPASK
UniProt Entry Name
PASK_HUMAN
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Product Notes
The PASK pask (Catalog #AAA9614180) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-PASK (Thr1165) Antibody reacts with Human, Mouse, Rat Predicted Reactivity: Pig (100%), Zebrafish (88%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (88%), Dog (88%), Chicken (100%) and may cross-react with other species as described in the data sheet. AAA Biotech's PASK can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Peptide ELISA (EIA). WB: 1:1000-3000 Peptide ELISA: 1:20,000-1:40,000. Researchers should empirically determine the suitability of the PASK pask for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PASK, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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