Rabbit anti-Human PARP10 Polyclonal Antibody | anti-PARP10 antibody

PARP10 Antibody (Center)

Cat. Num. AAA9217353

• Gene Names: PARP10; ARTD10
• Reactivity: Human
• Applications: Western Blot, ELISA
• Synonyms: PARP10; Polyclonal Antibody; PARP10 Antibody (Center); Poly [ADP-ribose] polymerase 10; PARP-10; 2.4.2.30; ADP-ribosyltransferase diphtheria toxin-like 10; ARTD10; Purified Rabbit Polyclonal Antibody (Pab); anti-PARP10 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Ig
• Specificity: This PARP10 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 565-597 amino acids from the Central region of human PARP10.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: 0.5 (varies by lot)
• Sequence Length: 1025

• Applicable Applications for anti-PARP10 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB~~1:2000
• Cellular Location: Nucleus, nucleolus. Cytoplasm. Note=Shuttles between the nuclear and cytoplasmic compartment. A subpopulation concentrates in the nucleolus during late G1/S phase
• Tissue Location: Highly expressed in spleen and thymus. Intermediate levels in liver, kidney, pancreas, prostate, testis, ovary, intestine, and leukocytes. Low expression in heart, brain, placenta, lung, skeletal muscle, and colon
• Antigen Type: Synthesized Peptide
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Anti-PARP10 Antibody (Center) at 1:2000 dilution + human spleen lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 110 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-PARP10 antibody

May play a role in cell proliferation. May be required for the maintenance of cell cycle progression.

References

Ota T.,et al.Nat. Genet. 36:40-45(2004). Suzuki Y.,et al.Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases. Yu M.,et al.Oncogene 24:1982-1993(2005). Chou H.Y.,et al.J. Biol. Chem. 281:15201-15207(2006). Choudhary C.,et al.Science 325:834-840(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 116248563
• NCBI GeneID: 84875
• UniProt Accession #: Q53GL7; Q8N2I0; Q8WV05; Q96CH7; Q96K72
• Molecular Weight: 109998 Da
• NCBI Official Full Name: Poly
• NCBI Official Synonym Full Names: poly(ADP-ribose) polymerase family member 10
• NCBI Official Symbol: PARP10
• NCBI Official Synonym Symbols: ARTD10
• NCBI Protein Information: poly [ADP-ribose] polymerase 10
• UniProt Protein Name: Poly [ADP-ribose] polymerase 10
• Protein Family: Poly [ADP-ribose] polymerase
• UniProt Gene Name: PARP10
• UniProt Synonym Gene Names: PARP-10; ARTD10
• UniProt Entry Name: PAR10_HUMAN

NCBI Description

Poly(ADP-ribose) polymerases (PARPs), such as PARP10, regulate gene transcription by altering chromatin organization by adding ADP-ribose to histones. PARPs can also function as transcriptional cofactors (Yu et al., 2005 [PubMed 15674325]).[supplied by OMIM, Mar 2008]

Uniprot Description

PARP10: May play a role in cell proliferation. May be required for the maintenance of cell cycle progression.

Protein type: EC 2.4.2.30; Transferase; Nucleolus

Chromosomal Location of Human Ortholog: 8q24.3

Cellular Component: cytoplasm; Golgi apparatus; nucleoplasm; nucleus

Molecular Function: NAD+ ADP-ribosyltransferase activity; protein binding

Biological Process: inhibition of NF-kappaB transcription factor; negative regulation of fibroblast proliferation; negative regulation of protein import into nucleus, translocation; negative regulation of viral genome replication; protein amino acid ADP-ribosylation